Invitrogen

DOK5 Polyclonal Antibody

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DOK5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DOK5 in A549 cells using a DOK5 polyclonal antibody (Product # PA5-97676) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

DOK5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of DOK5 in paraffin embedded human tonsil tissue using a DOK5 polyclonal antibody (Product # PA5-97676) at a dilution of 1:100.

DOK5 Antibody in Western Blot (WB) — Western Blot analysis of DOK5 using a DOK5 Polyclonal antibody (Product # PA5-97676) at a concentration of 2 µg/mL. Positive WB detected in Mouse kidney tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:15,000 dilution. Observed band size: 36 kDa.

产品信息

PA5-97676

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Docking protein 5 protein (1-198AA)

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

4 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

RRID

AB_2812291

靶标信息

The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP.

仅用于科研。不用于诊断过程。未经明确授权不得转售。