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DRB4/Double-stranded RNA-binding protein 4 Polyclonal Antibody
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DRB4/Double-stranded RNA-binding protein 4 Antibody (AS15-3104) in WB
100 µg of total protein from Arabidopsis thaliana, drb 4, and 35 S-DRB 4 leaves extracted with isolation buffer (50 m M Tris-HCl, p H 7.5,10% glycerol, 150 m M Na Cl, 10 m M Mg Cl 2,5 m M EDTA, 5 m M DTT, and 1 X protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and denatured by boiling at 100°C. The proteins were separated on 10% SDS-PAGE and blotted 1.5 hrs to PVDF membrane using wet transfer. Bl... View More
产品信息
AS15-3104
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana DRB4 sequence.
偶联物
形式
Lyophilized
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
DRB4 is a protein-coding gene within the major histocompatibility complex class II region and encodes an HLA-DR beta chain that pairs with the invariant HLA-DRA alpha chain to form a cell-surface, membrane-anchored heterodimer on professional antigen-presenting cells. The encoded beta chain is synthesized as a type I membrane glycoprotein precursor (approximately 26-28 kDa) with an N-terminal signal peptide, two extracellular domains that create the peptide-binding platform and Ig-like domain, a single-pass transmembrane helix, and a short cytoplasmic tail; gene organization classically places the leader sequence in exon 1, the extracellular domains in exons 2-3, the transmembrane segment in exon 4, and the cytoplasmic tail in exon 5 (with additional untranslated or alternatively annotated exons reported on alternate loci). Functionally, the DRB4-containing HLA-DR complex binds peptides generated mainly in the endocytic/lysosomal pathway from extracellular or vesicular proteins and displays them at the cell surface for surveillance by CD4 T cells, thereby shaping adaptive immune responses; extensive allelic polymorphism in the beta chain is a key determinant of peptide-binding specificity and underlies its importance in histocompatibility matching and disease association studies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATTIF3K1; double-stranded-RNA-binding protein 4
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