Invitrogen

DRD2 Polyclonal Antibody

查看其他25个DRD2抗体

问题与答案

问题与答案

抗体检测数据 (8)

DRD2 Antibody in Western Blot (WB)

DRD2 Antibody in Western Blot (WB)

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DRD2 Antibody (PA5-71958) in WB

Western blot analysis of DRD2 in various lysates. Samples were incubated with DRD2 polyclonal antibody (Product # PA5-71958) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: human brain lysate; Lane 2: human cerebellum lysate; Lane 3: HT-1080 whole cell lysate; Lane 4: K562 whole cell lysate. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DRD2 Antibody in Western Blot (WB)

DRD2 Antibody in Western Blot (WB)

DRD2 Antibody in Western Blot (WB)

DRD2 Antibody in Western Blot (WB)

DRD2 Antibody in Western Blot (WB)

DRD2 Antibody in Western Blot (WB)

DRD2 Antibody in Western Blot (WB)

DRD2 Antibody in Western Blot (WB)

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DRD2 Antibody in Western Blot (WB) — Western blot analysis of DRD2 in various lysates. Samples were incubated with DRD2 polyclonal antibody (Product # PA5-71958) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: human brain lysate; Lane 2: human cerebellum lysate; Lane 3: HT-1080 whole cell lysate; Lane 4: K562 whole cell lysate. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DRD2 Antibody in Western Blot (WB) — Western blot analysis of DRD2 in the following samples: Lane 1: human brain lysate, Lane 2: human cerebellum lysate, Lane 3: HT-1080 whole cell lysate, Lane 4: K562 whole cell lysate. 20 µg of protein per lane was used. Samples were first blocked with 5% NFDM/TBST, incubated with DRD2 polyclonal antibody (Product # PA5-71958) using a dilution of 1:2000 followed by a goat anti-rabbit IgG H and L (HRP) secondary antibody with a dilution of 1:10,000.

DRD2 Antibody in Western Blot (WB) — Western blot analysis of DRD2 in various lysates. Samples were incubated with DRD2 polyclonal antibody (Product # PA5-71958) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: human brain lysates; Lane 2: K562 whole cell lysates; Lane 3: human cerebellum lysates. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DRD2 Antibody in Western Blot (WB) — Western blot analysis of DRD2 in HT-1080 whole cell lysate. Samples were incubated with DRD2 polyclonal antibody (Product # PA5-71958) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DRD2 Antibody in Western Blot (WB) — Western blot analysis of DRD2 in K562 whole cell lysate. Samples were incubated with DRD2 polyclonal antibody (Product # PA5-71958) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DRD2 Antibody in Western Blot (WB) — Western blot analysis of DRD2 in K562 whole cell lysate. Samples were incubated with DRD2 polyclonal antibody (Product # PA5-71958) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DRD2 Antibody in Western Blot (WB) — Western blot analysis of DRD2 in Human brain lysate. Samples were incubated with DRD2 polyclonal antibody (Product # PA5-71958) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DRD2 Antibody in Western Blot (WB) — Western blot analysis of DRD2 in Human brain lysate. Samples were incubated with DRD2 polyclonal antibody (Product # PA5-71958) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

产品信息

PA5-71958

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 307-336 amino acids from the C-terminal region of human DRD2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2717812

靶标信息

The DRD2 Dopamine Receptor is involved in the neuromodulation of appetitive behaviors. DRD2 may be linked to alcoholism and other substance-use disorders. Mutations in DRD2 in mice lead to the suppression of reward behavior with morphine and to the reduction of spontaneous movements, a phenotype resembling Parkinsons disease. Three alternatively spliced isoforms have been identified. DRD2 has been reported to be expressed in various regions of the brain, as well as in aorta, artery, eye, and spinal cord. ESTs have been isolated from normal pituitary and in brain, colon, lung, pancreas, and skeletal muscle cancer libraries.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: D(2) dopamine receptor; Dopamine 2 receptor; Dopamine D2 receptor; dopamine receptor D2 isoform; seven transmembrane helix receptor

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基因别名: D2DR; D2R; DRD2

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UniProt ID: (Human) P14416

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Entrez Gene ID: (Human) 1813

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dopamine neurotransmitter receptor activity, coupled via Gi/Go adrenergic receptor activity protein binding drug binding dopamine binding ionotropic glutamate receptor binding identical protein binding protein homodimerization activity protein heterodimerization activity G-protein coupled receptor

temperature homeostasis response to hypoxia synaptic transmission, dopaminergic response to amphetamine neurological system process involved in regulation of systemic arterial blood pressure regulation of heart rate regulation of sodium ion transport G-protein coupled receptor internalization positive regulation of neuroblast proliferation positive regulation of receptor internalization negative regulation of adenylate cyclase activity adenylate cyclase-inhibiting dopamine receptor signaling pathway neuron-neuron synaptic transmission axonogenesis synapse assembly sensory perception of smell long-term memory grooming behavior locomotory behavior adult walking behavior feeding behavior protein localization negative regulation of cell proliferation associative learning visual learning response to light stimulus response to toxic substance response to iron ion regulation of dopamine secretion response to inactivity Wnt signaling pathway striatum development orbitofrontal cortex development cerebral cortex GABAergic interneuron migration adenohypophysis development negative regulation of cell migration peristalsis regulation of cAMP metabolic process auditory behavior activation of protein kinase activity regulation of synaptic transmission, GABAergic positive regulation of cytokinesis circadian regulation of gene expression negative regulation of dopamine secretion response to histamine response to nicotine positive regulation of urine volume positive regulation of renal sodium excretion positive regulation of multicellular organism growth response to cocaine negative regulation of circadian sleep/wake cycle, sleep dopamine metabolic process response to drug regulation of potassium ion transport response to morphine pigmentation regulation of phosphoprotein phosphatase activity positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of blood pressure negative regulation of innate immune response positive regulation of transcription from RNA polymerase II promoter phosphatidylinositol metabolic process negative regulation of insulin secretion acid secretion behavioral response to cocaine behavioral response to ethanol regulation of long-term neuronal synaptic plasticity response to axon injury branching morphogenesis of a nerve arachidonic acid secretion negative regulation of protein secretion release of sequestered calcium ion into cytosol negative regulation of cytosolic calcium ion concentration regulation of dopamine uptake involved in synaptic transmission positive regulation of dopamine uptake involved in synaptic transmission regulation of synapse structural plasticity negative regulation of protein kinase B signaling negative regulation of synaptic transmission, glutamatergic positive regulation of growth hormone secretion prepulse inhibition phospholipase C-activating dopamine receptor signaling pathway negative regulation of dopamine receptor signaling pathway positive regulation of ERK1 and ERK2 cascade adenylate cyclase-activating adrenergic receptor signaling pathway regulation of locomotion involved in locomotory behavior chemical synaptic transmission, postsynaptic positive regulation of glial cell-derived neurotrophic factor secretion positive regulation of long-term synaptic potentiation negative regulation of voltage-gated calcium channel activity