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DYKDDDDK Tag Antibody (701629RB) in WB
Western blot was performed using DYKDDDDK tag Recombinant Rabbit monoclonal antibody (8H8L17), Biotin (Product # 701629RB) by loading 30 µg modified whole cell extracts (1% SDS) of untransfected and transiently transfected HEK-293E lysates: Untransfected (Lane 1), empty vector control (Lane 2), HA-H3-DYKDDDDK (Lane 3), H3.3K4M-DYKDDDDK (Lane 4), H3.3K9M-DYKDDDDK (Lane 5), H3.3G34W-DYKDDDDK (Lane 6), DYKDDDDK-p65-HA (Lane 7), and 25 ng of Positope (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). ... View More
产品信息
701629RB
产品规格
种属反应
Tag
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
8H8L17
抗原
Synthetic peptide (DYKDDDDK)
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
DYKDDDDK Tag recombinant rabbit monoclonal antibody conjugated with Biotin specifically recognizes DYKDDDDK Tag at the C-terminus but not N-terminus of proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to a larger rabbit immune repertoire.
This antibody has been tested in western blot. The clone is reported to also work in immunoprecipitation (IP) assay, and it is recommended that the antibody be carefully titrated for optimal performance in this assay for best results.
靶标信息
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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