Invitrogen

Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), eBioscience™

Product Image Gallery

Dendritic Cell Marker DCIR2 Antibody in Flow Cytometry (Flow) — Staining of BALB/c splenocytes with 0.25 µg of Rat IgG2b K Isotype Control Purified (Product # 14-4031-82) (open histogram) or 0.25 µg of Anti-Mouse Dendritic Cell Marker DCIR2 Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis.

产品信息

14-5884-82

应用	建议稀释比	已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	-	查看3篇已发表文章 3篇已发表文章
免疫组化（冰冻） (IHC (F))	Assay-Dependent	查看1篇已发表文章 1篇已发表文章
免疫细胞化学 (ICC/IF)	-	查看1篇已发表文章 1篇已发表文章
流式细胞分析 (Flow)	1 µg/test	查看3篇已发表文章 3篇已发表文章
功能检测 (FN)	Assay-Dependent	-

产品规格
种属反应	Mouse
已发表种属	Mouse
宿主/亚型	Rat / IgG2b, kappa
分类	Monoclonal
类型	Antibody
克隆号	33D1
偶联物	Unconjugated PE PerCP-eFluor 710
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	4° C
RRID	AB_467676

产品详细信息

Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

Applications Reported: This 33D1 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistochemical staining. It has also been reported in complement-mediated cytotoxicity.

Applications Tested: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For detection of purified 33D1 it is recommended not to use goat anti-rat due to its low affinity for 33D1. Rather, we recommend using biotin mouse anti-rat (cat. 13-4813).

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Protective role for Toll-like receptor-9 in the development of atherosclerosis in apolipoprotein E-deficient mice.

Arteriosclerosis, thrombosis, and vascular biology

Koulis C,Chen YC,Hausding C,Ahrens I,Kyaw TS,Tay C,Allen T,Jandeleit-Dahm K,Sweet MJ,Akira S,Bobik A,Peter K,Agrotis A

14-5884 was used in Immunohistochemistry to investigate whether TLR9 has a role in the development of atherosclerosis in apolipoprotein E-deficient mice.

Sat Mar 01 00:00:00 EST 2014

Colon Macrophages Polarized by Commensal Bacteria Cause Colitis and Cancer through the Bystander Effect.

Translational oncology

Yang Y,Wang X,Huycke T,Moore DR,Lightfoot SA,Huycke MM

14-5884 was used in Immunohistochemistry on paraffin embedded tissues to investigate whether colon macrophages are key effectors for the bystander effect.

Wed Oct 23 00:00:00 EDT 2013

Regulation of dendritic cell differentiation in bone marrow during emergency myelopoiesis.

Journal of immunology (Baltimore, Md. : 1950)

Liu H,Zhou J,Cheng P,Ramachandran I,Nefedova Y,Gabrilovich DI

14-5884 was used as a Control in experiments to demonstrate that dendritic cell differentiation during induced myelopoiesis can be differentially regulated by the Notch ligands Jagged-1 and Delta-like ligand 1.

Thu Aug 15 00:00:00 EDT 2013

Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice.

BMC gastroenterology

Maldonado Galdeano C,Novotny Núñez I,de Moreno de LeBlanc A,Carmuega E,Weill R,Perdigón G

14-5884 was used in Immunofluorescence to evaluate the effects of a probiotic fermented milk as a complement of a re-nutrition diet and for its protection against Salmonella.

Thu May 26 00:00:00 EDT 2011

Comparable T helper 1 (Th1) and CD8 T-cell immunity by targeting HIV gag p24 to CD8 dendritic cells within antibodies to Langerin, DEC205, and Clec9A.

Proceedings of the National Academy of Sciences of the United States of America

Idoyaga J,Lubkin A,Fiorese C,Lahoud MH,Caminschi I,Huang Y,Rodriguez A,Clausen BE,Park CG,Trumpfheller C,Steinman RM

Tue Feb 08 00:00:00 EST 2011

Targeting Stat3 in the myeloid compartment drastically improves the in vivo antitumor functions of adoptively transferred T cells.

Cancer research

Herrmann A,Kortylewski M,Kujawski M,Zhang C,Reckamp K,Armstrong B,Wang L,Kowolik C,Deng J,Figlin R,Yu H

14-5884 was used in Flow cytometry/Cell sorting to study the effects of inhibiting the signal transducer and activator of transcription 3 (Stat3) on antitumour functions.

Fri Oct 01 00:00:00 EDT 2010

Effect of the administration of a fermented milk containing Lactobacillus casei DN-114001 on intestinal microbiota and gut associated immune cells of nursing mice and after weaning until immune maturity.

BMC immunology

de Moreno de LeBlanc A,Dogi CA,Galdeano CM,Carmuega E,Weill R,Perdigón G

14-5884 was used in Immunohistochemistry on paraffin embedded tissues to evaluate the effect of the administration of a probiotic fermented milk on the intestinal bacterial population and on parameters of the gut immune system.

Fri Jun 13 00:00:00 EDT 2008

Combined treatment with triptolide and rapamycin prolongs graft survival in a mouse model of cardiac transplantation.

Transplant international : official journal of the European Society for Organ Transplantation

Liu Y,Chen Y,Liu FQ,Lamb JR,Tam PK

14-5884 was used in Immunohistochemistry to study the effects of triptolide and rapamycin combination therapy on the survival of cardiac allografts.

Thu May 01 00:00:00 EDT 2008

Differential antigen processing by dendritic cell subsets in vivo.

Science (New York, N.Y.)

Dudziak D,Kamphorst AO,Heidkamp GF,Buchholz VR,Trumpfheller C,Yamazaki S,Cheong C,Liu K,Lee HW,Park CG,Steinman RM,Nussenzweig MC

Fri Jan 05 00:00:00 EST 2007

生物信息学

蛋白别名: C-type lectin domain family 4, member a4; DC Marker; dendritic cell inhibitory receptor 2

基因别名: Dcir2

Entrez Gene ID: (Mouse) 474145

分子生物学功能: membrane traffic protein

功能

biological_process

参与通路

carbohydrate derivative binding

Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), eBioscience™

产品信息

14-5884-82

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

功能检测 (FN)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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