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Invitrogen
| 应用 | 建议稀释比 |
|---|---|
免疫印迹 (WB) |
1:5,000-1:20,000 |
免疫组化 (IHC) |
1:50-1:2,000 |
免疫细胞化学 (ICC/IF) |
1:50-1:2,000 |
流式细胞分析 (Flow) |
1:25 - 1:100 |
免疫沉淀 (IP) |
Assay-dependent |
| 产品规格 | |
|---|---|
种属反应 |
Mouse |
宿主/亚型 |
Donkey / IgG |
分类 |
Polyclonal |
类型 |
Secondary Antibody |
抗原 |
Mouse IgG-heavy and light chain |
偶联物 |
DyLight™ 488
|
激发/发射光谱 |
查看光谱
|
形式 |
Liquid |
浓度 |
0.5 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
PBS with 0.2% BSA |
内含物 |
0.09% sodium azide |
保存条件 |
4° C |
RRID |
AB_2556746 |
靶标 |
IgG |
交叉吸附 |
Against bovine, chicken, goat, human, rabbit, rat and sheep |
抗体形式 |
Whole Antibody |
This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, goat, human, rabbit, rat and sheep.
For Western blot applications, 5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.
Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
