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| 应用 | 建议稀释比 |
|---|---|
免疫细胞化学 (ICC/IF) |
1:500-1:20,000 |
流式细胞分析 (Flow) |
1:500-1:20,000 |
| 产品规格 | |
|---|---|
种属反应 |
Rabbit |
宿主/亚型 |
Donkey / IgG |
分类 |
Polyclonal |
类型 |
Secondary Antibody |
抗原 |
Gamma Immunoglobins Heavy and Light chains |
偶联物 |
FITC
|
激发/发射光谱 |
查看光谱
|
形式 |
Liquid |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2, with 1% BSA |
内含物 |
0.05% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
RRID |
AB_2534698 |
靶标 |
IgG |
抗体形式 |
Whole Antibody |
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents
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