Invitrogen

Dynactin 1 Monoclonal Antibody (6B11)

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Dynactin 1 Antibody in Western Blot (WB) — Western blot was performed using Anti-Dynactin 1 Monoclonal Antibody (6B11) (Product # MA5-18308) and a 141 kDa band corresponding to DCTN1 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1) and HEK-293 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1750 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Dynactin 1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Dynactin 1 in HeLa cells. Samples were incubated with Dynactin 1 monoclonal antibody (Product # MA5-18308).

产品信息

MA5-18308

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000-1:1,750	-
免疫沉淀 (IP)	1:50-1:200	-

产品规格
种属反应	Human
宿主/亚型	Mouse / IgG2b
分类	Monoclonal
类型	Antibody
克隆号	6B11
抗原	Synthetic peptide corresponding to the N-terminal of human Dynactin 1 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.2, with 50% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2539681

产品详细信息

This antibody detects endogenous levels of DCTN1 at a molecular weight of 150 kDa and does not cross-react with related proteins.

Purity is >95% by SDS-PAGE.

靶标信息

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 150 kDa dynein-associated polypeptide; DAP 150; DAP-150; DAP150; DCTN 1; DP 150; DP-150; DP150; dynactin 1 (p150, glued homolog, Drosophila); Dynactin subunit 1; Dynactin1; HMN7B; OTTHUMP00000202491; p135; p150 glued; p150 glued homolog; p150-glued; p150glued

基因别名: DAP-150; DCTN1; DP-150; P135

UniProt ID: (Human) Q14203

Entrez Gene ID: (Human) 1639

分子生物学功能: chaperone

功能

G2/M transition of mitotic cell cycle ER to Golgi vesicle-mediated transport mitotic nuclear division nervous system development transport along microtubule antigen processing and presentation of exogenous peptide antigen via MHC class II melanosome transport IRE1-mediated unfolded protein response retrograde transport, endosome to Golgi

参与通路

motor activity protein binding dynein binding

Dynactin 1 Monoclonal Antibody (6B11)

产品信息

MA5-18308

免疫印迹 (WB)

免疫沉淀 (IP)

种属反应

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

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