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EB1 Antibody (PA5-96188) in WB
Western blot analysis of EB1 in extracts of various cell lines. Samples were incubated with EB1 Polyclonal antibody (Product # PA5-96188) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-96188
产品规格
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 134-268 of human MAPRE1.
Conjugate
Unconjugated
形式
Liquid
浓度
1.96 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2807990
Product Specific Information
Immunogen sequence: ETAVAPSLVA PALNKPKKPL TSSSAAPQRP ISTQRTAAAP KAGPGVVRKN PGVGNGDDEA AELMQQVNVL KLTVEDLEKE RDFYFGKLRN IELICQENEG ENDPVLQRIV DILYATDEGF VIPDEGGPQE EQEEY; Positive Samples: B-cell, U-87MG, LO2, HT-29, NIH/3T3, Mouse lung, Mouse spleen, Rat heart; Cellular Location: Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Target Information
EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. The MAPRE genes encode the EB1 family proteins. The human MAPRE genes MAPRE1, MAPRE2, and MAPRE3 code for EB1, RP1, and EBF3 proteins, respectively. EB1 family members appear to be expressed ubiquitously. The EB1 (MAPRE1) gene, at 20q11. 2, is fused to MLL in an adult patient with pro-B acute lymphoblastic leukemia. EB1 is a microtubule-associated protein that interacts with the colorectal adenomatous polyposis coli tumor-suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: adenomatosis polyposis coli binding protein Eb1; adenomatous polyposis coli-binding protein EB1; APC-binding protein EB1; EB1; End-binding protein 1; MGC117374; MGC129946; Microtubule-associated protein RP/EB family member 1; microtubule-associated protein, RP/EB family, member 1
Gene Aliases: 5530600P05Rik; AI462499; AI504412; AW260097; BIM1p; D2Ertd459e; EB1; MAPRE1
UniProt ID: (Human) Q15691, (Rat) Q66HR2, (Mouse) Q61166
Entrez Gene ID: (Human) 22919, (Rat) 114764, (Mouse) 13589
Molecular Function:
non-motor microtubule binding protein
G2/M transition of mitotic cell cycle
sister chromatid cohesion
mitotic nuclear division
cell proliferation
positive regulation of cell migration
negative regulation of microtubule polymerization
protein localization to microtubule
cell division
cell-cell adhesion
positive regulation of microtubule plus-end binding
negative regulation of microtubule binding
regulation of microtubule polymerization
cell cycle
regulation of microtubule plus-end binding
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