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Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4 C, not refrozen, and preferably used the same day.
In immunoelectrophoresis use 2 µL serum or equivalent against 120 µL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µL antiserum in 3 mm diameter center well and 2 µL serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components.
Epstein Barr Virus nuclear antigen 3C (EBNA-3C) is a latent viral nuclear protein expressed in EBV transformed lymphoblastic cell lines and is essential for EBV mediated transformation of these cell lines. This viral nuclear protein is essential for EBV mediated positive immunoblastic lymphomas, and functions as a transcription factor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: EBNA-3C