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Invitrogen
EEF2 Polyclonal Antibody
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EEF2 Antibody (PA5-19613) in ICC/IF
Immunofluorescent staining of HeLa using Product # PA5-19613, anti-EEF2 antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 20 minutes and exposed to the primary antibody at a concentration of 5 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAP... View More
产品信息
PA5-19613
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human EEF2.
偶联物
形式
Liquid
浓度
0.4 mg/mL
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with chicken, hamster, cow, Xenopus laevis and zebrafish based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
靶标信息
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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