Invitrogen

EGF Polyclonal Antibody

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View all (31) EGF antibodies
Datasheet
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Datasheet
Protocols
Tech Support

Product Details

PA5-96195

Applications
Tested Dilution
Publications

免疫印迹 (WB)

1:500-1:2,000
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点杂交 (DB)

1:500-1:2,000
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产品规格

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

分类

Polyclonal

类型

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 971-1023 of human EGF.

Conjugate

Unconjugated

形式

Liquid

浓度

1.29 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2807997

Product Specific Information

Positive Samples: Mouse kidney; Cellular Location: Membrane, Single-pass type I membrane protein

Target Information

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Bioinformatics

Protein Aliases: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); Urogastrone

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Gene Aliases: AI790464; EGF; HOMG4; URG

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UniProt ID: (Human) P01133, (Mouse) P01132

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Entrez Gene ID: (Human) 1950, (Mouse) 13645

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Molecular Function: transmembrane signal receptor

Function(s)
MAPK cascade activation of MAPKK activity activation of MAPK activity angiogenesis platelet degranulation DNA replication signal transduction activation of transmembrane receptor protein tyrosine kinase activity epidermal growth factor receptor signaling pathway STAT protein import into nucleus positive regulation of cell proliferation positive regulation of peptidyl-threonine phosphorylation regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation positive regulation of cerebellar granule cell precursor proliferation positive regulation of catenin import into nucleus ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway positive regulation of phosphorylation positive regulation of DNA binding positive regulation of MAP kinase activity positive regulation of GTPase activity Wnt signaling pathway involved in dorsal/ventral axis specification positive regulation of epidermal growth factor-activated receptor activity positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling branching morphogenesis of an epithelial tube negative regulation of secretion canonical Wnt signaling pathway mammary gland alveolus development ERK1 and ERK2 cascade regulation of calcium ion import negative regulation of cholesterol efflux positive regulation of hyaluronan biosynthetic process regulation of protein localization to cell surface positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of cell motility regulation of protein secretion regulation of peptidyl-tyrosine phosphorylation regulation of protein transport
Process(es)
protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding calcium ion binding protein binding growth factor activity Wnt-protein binding transmembrane receptor protein tyrosine kinase activator activity Wnt-activated receptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity
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