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Invitrogen
EGFR Monoclonal Antibody (199.12)
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EGFR Antibody (AHR5072) in ICC/IF
Immunofluorescent analysis of EGFR (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Epidermal Growth Factor Receptor monoclonal antibody (Product # AHR5072) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at r... View More
产品信息
AHR5072
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Chicken,
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
199.12
抗原
The extracellular domain of human recombinant EGFR protein, purified.
偶联物
形式
Liquid
浓度
0.2 mg/mL
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
AHR5072 reacts with an extracellular domain of EGFR. The molecular weight of the antigen is 170kDa (wild type) and 145kDa (vIII variant). This antibody does not block the binding of EGF to EGFR.
靶标信息
EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
