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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • EGFR Antibodies

          Invitrogen

          EGFR Monoclonal Antibody (225)

          高级验证
          1个已发表图片
          29 篇参考文献
          查看其他187个EGFR抗体

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          说明书
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          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite EGFR Monoclonal Antibody (225)

          • 抗体检测数据 (9)
          • 已发表图片 (1)
          • 高级验证 (5)
          EGFR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          EGFR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 15

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          EGFR Antibody (MA5-12880) in ICC/IF

          Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EGFR Mouse monoclonal antibody (Product # MA5-12880) at 5 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          EGFR Antibody in Immunocytochemistry (ICC/IF)
          EGFR Antibody in Immunocytochemistry (ICC/IF)
          EGFR Antibody in Immunocytochemistry (ICC/IF)
          EGFR Antibody in Immunocytochemistry (ICC/IF)
          EGFR Antibody in Immunocytochemistry (ICC/IF)
          EGFR Antibody in Immunocytochemistry (ICC/IF)
          EGFR Antibody in Immunocytochemistry (ICC/IF)
          EGFR Antibody in Immunocytochemistry (ICC/IF)
          EGFR Antibody in Immunoprecipitation (IP)
          EGFR Antibody in Western Blot (WB)
          EGFR Antibody
          EGFR Antibody
          EGFR Antibody
          EGFR Antibody
          EGFR Antibody

          产品信息

          MA5-12880

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          -
          查看4篇已发表文章 4篇已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          5 µg/mL
          查看10篇已发表文章 10篇已发表文章

          流式细胞分析 (Flow)

          -
          查看3篇已发表文章 3篇已发表文章

          免疫沉淀 (IP)

          2 µg/mL
          查看1篇已发表文章 1篇已发表文章

          中和实验 (Neu)

          -
          查看9篇已发表文章 9篇已发表文章

          凝胶阻滞分析 (GS)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human, Non-human primate

          已发表种属

          Human, Mouse

          宿主/亚型

          Mouse / IgG1

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          225

          抗原

          Purified EGFR from A431 cells
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Protein G

          保存液

          PBS, pH 7.4, with 0.2% BSA

          内含物

          0.09% sodium azide

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10982150

          产品详细信息

          MA5-12880 targets Epidermal Growth Factor Receptor in IP and IF applications and shows reactivity with Human and Non-human primate samples.

          The MA5-12880 immunogen is purified EGFR from A431 cells.

          靶标信息

          EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone

          查看更多 收起

          基因别名: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61

          查看更多 收起

          UniProt ID: (Human) P00533

          查看更多 收起

          Entrez Gene ID: (Human) 1956

          查看更多 收起

          功能
          chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion transmembrane signal receptor
          参与通路
          MAPK cascade activation of MAPKK activity cell morphogenesis ossification embryonic placenta development positive regulation of protein phosphorylation hair follicle development response to stress signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway activation of phospholipase C activity salivary gland morphogenesis learning or memory cell proliferation positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion peptidyl-tyrosine phosphorylation cerebral cortex cell migration positive regulation of cell growth positive regulation of cell migration positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle positive regulation of catenin import into nucleus ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway negative regulation of protein catabolic process positive regulation of phosphorylation activation of phospholipase A2 activity by calcium-mediated signaling negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of GTPase activity positive regulation of nitric oxide biosynthetic process positive regulation of DNA repair positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation digestive tract morphogenesis positive regulation of epithelial cell proliferation regulation of peptidyl-tyrosine phosphorylation regulation of nitric-oxide synthase activity protein insertion into membrane positive regulation of protein kinase B signaling morphogenesis of an epithelial fold eyelid development in camera-type eye response to UV-A positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to epidermal growth factor stimulus cellular response to estradiol stimulus positive regulation of production of miRNAs involved in gene silencing by miRNA regulation of cell motility
          It has to be done as per old AB suggested Products section.
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