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Invitrogen
EHD2 Polyclonal Antibody
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EHD2 Antibody (PA5-18303) in ICC/IF
Immunocytochemistry analysis of EHD2 using EHD2 Polyclonal Antibody (Product # PA5-18303) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
产品信息
PA5-18303
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide sequence (CRLVPPSKRRHKGSA) corresponding to the C-terminus amino acids of EHD2 (aa 533-543).
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
靶标信息
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EH domain-containing protein 2; EH-domain containing 2; FLJ96617; PAST homolog 2
基因别名: EHD2; PAST2
UniProt ID: (Human) Q9NZN4
Entrez Gene ID: (Human) 30846
