Proteintech

EMG1 Polyclonal Antibody

4 篇参考文献

查看其他5个EMG1抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (6)

EMG1 Antibody in Immunocytochemistry (ICC/IF)

EMG1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

EMG1 Antibody (11965-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 11965-1-AP (EMG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

EMG1 Antibody in Immunocytochemistry (ICC/IF)

EMG1 Antibody in Western Blot (WB)

EMG1 Antibody in Western Blot (WB)

EMG1 Antibody in Western Blot (WB)

EMG1 Antibody in Western Blot (WB)

EMG1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

EMG1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 11965-1-AP (EMG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

EMG1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EMG1 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EMG1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

EMG1 Antibody in Western Blot (WB) — WB result of EMG1 antibody (Product # 11965-1-AP) 1:20,000, incubated at room temperature for 1.5 hours with sh-Control and sh-EMG1 transfected HEK-293 cells.

EMG1 Antibody in Immunoprecipitation (IP) — IP result of anti-EMG1 (IP:11965-1-AP, 4ug; Detection:11965-1-AP 1:400) with HeLa cells lysate 1080ug.

Immunogen image

EMG1 Polyclonal Antibody

Immunogen Image EMG1 Polyclonal Antibody

产品信息

11965-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2400

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免疫细胞化学 (ICC/IF)

1:20-1:200

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免疫沉淀 (IP)

0.5-4.0 µg

-

其他PubMed (Misc)

-

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不适用 (N/A)

-

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

EMG1 Fusion Protein Ag2575 (1-244 aa encoded by BC055314)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.13 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: MAAPSDGFK PRERSGGEQA QDWDALPPKR PRLGAGNKIG GRRLIVVLEG ASLETVKVGK TYELLNCDKH KSILLKNGRD PGEARPDITH QSLLMLMDSP LNRAGLLQVY IHTQKNVLIE VNPQTRIPRT FDRFCGLMVQ LLHKLSVRAA DGPQKLLKVI KNPVSDHFPV GCMKVGTSFS IPVVSDVREL VPSSDPIVFV VGAFAHGKVS VEYTEKMVSI SNYPLSAALT CAKLTTAFEE VWGVI (1-244 aa encoded by BC055314)

靶标信息

EMG1 involved in 40S ribosomal subunit biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 18S rRNA (pseudouridine(1248)-N1)-methyltransferase; 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1; 18S rRNA Psi1248 methyltransferase; EMG1 N1-specific pseudouridine methyltransferase; EMG1 nucleolar protein homolog; essential for mitotic growth 1; gene rich cluster, C2f; nucleolar essential protein 1; Nucleolar protein EMG1 homolog; probable ribosome biogenesis protein NEP1; Protein C2f; Ribosomal RNA small subunit methyltransferase NEP1; Ribosome biogenesis protein NEP1

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基因别名: C2F; EMG1; Grcc2f; NEP1

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UniProt ID: (Human) Q92979, (Mouse) O35130

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Entrez Gene ID: (Human) 10436, (Mouse) 14791, (Rat) 312706

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RNA binding protein binding rRNA binding poly(A) RNA binding rRNA (pseudouridine) methyltransferase activity rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity methyltransferase

maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA processing ribosomal small subunit biogenesis rRNA base methylation blastocyst development nucleologenesis rRNA methylation methylation ribosome biogenesis