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Invitrogen
ERAS Polyclonal Antibody
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ERAS Antibody (PA5-81382) in WB
Western Blot using ERAS Polyclonal Antibody (Product # PA5-81382) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: Mouse Lung tissue lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 28 kDa.
产品信息
PA5-81382
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to the center region of the Mouse ERAS
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Mouse ERAS.
靶标信息
The human ERAS gene was initially reported as a processed pseudogene (HRasp, Ha-Ras2), with several base substitutions in coding regions, but was more recently described as potentially encoding a functional human ERAS protein. The ERAS oncogene supports the tumorigenic growth of embryonic stem cells by producing a constitutively active Ras protein. ERAS protein has less than 50% amino acid identity with H-Ras, another member of Ras family but the key domains essential for Ras function are highly conserved. The protein also contains amino-acid residues identical to those present in active mutants of Ras and causes oncogenic transformation in NIH 3T3 cells. Human ERAS gene is localized in the chromosomal region Xp11.23.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E-Ras; Embryonic stem cell-expressed Ras; GTPase ERas; small GTPase E-Ras
基因别名: ecat5; Eras; Ha-Ras2; HRAS2; HRasp
UniProt ID: (Mouse) Q7TN89
Entrez Gene ID: (Mouse) 353283
