Invitrogen

EZH2 Monoclonal Antibody (H.547.3)

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EZH2 Antibody (MA5-15101) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF7 (Lane 2), HCT 116 (Lane 3), SW480 (Lane 4), Caco-2 (Lane 5), T98G (Lane 6), PC-3 (Lane 7), DU 145 (Lane 8), Jurkat (Lane9) and Hep G2 (Lane 10). The blot was probed with Anti-EZH2 Monoclonal Antibody (H.547.3) (Product # MA5-15101, 1:1000 dilution) and detected by chemiluminescence using Goat anti Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). An 86 kDa band corresponding to... View More

EZH2 Antibody in Immunocytochemistry (ICC/IF)

EZH2 Antibody in Immunohistochemistry (IHC)

EZH2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

EZH2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with EZH2 siRNA and reduction of signal was observed in Western Blot using EZH2 Monoclonal Antibody (H.547.3) (Product # MA5-15101). {KD}

产品信息

MA5-15101

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

H.547.3

抗原

Ezh2 recombinant protein containing human Ezh2 residues 351-453

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

403 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11004378

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Neuroprotective effects of GSK-343 in an in vivo model of MPTP-induced nigrostriatal degeneration.

Journal of neuroinflammation

Mannino D,Scuderi SA,Casili G,Bova V,Cucinotta L,Lanza M,Filippone A,Esposito E,Paterniti I

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Western Blot

Fri Jun 30 00:00:00 UTC 2023

Serum-Derived Small Extracellular Vesicles From Diabetic Mice Impair Angiogenic Property of Microvascular Endothelial Cells: Role of EZH2.

Journal of the American Heart Association

Cheng Z,Naga Srikanth Garikipati V,Truongcao MM,Cimini M,Huang G,Wang C,Benedict C,Gonzalez C,Mallaredy V,Goukassian DA,Verma SK,Kishore R

MA5-15101 was used in Immunohistochemistry to study the role of serum-derived small extracellular vesicles (ssEVs) in diabetes mellitus-impaired microvascular endothelial cell function.

Tue May 18 00:00:00 UTC 2021

Serum-Derived Small Extracellular Vesicles From Diabetic Mice Impair Angiogenic Property of Microvascular Endothelial Cells: Role of EZH2.

Journal of the American Heart Association

Cheng Z,Naga Srikanth Garikipati V,Truongcao MM,Cimini M,Huang G,Wang C,Benedict C,Gonzalez C,Mallaredy V,Goukassian DA,Verma SK,Kishore R

MA5-15101 was used in Immunohistochemistry to study the role of serum-derived small extracellular vesicles (ssEVs) in diabetes mellitus-impaired microvascular endothelial cell function.

Tue May 18 00:00:00 UTC 2021

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Cell death & disease

Zhang T,Yang L,Ke Y,Lei J,Shen S,Shao S,Zhang C,Zhu Z,Dang E,Wang G

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Immunohistochemistry

Sat Oct 03 00:00:00 UTC 2020

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Cell death & disease

Zhang T,Yang L,Ke Y,Lei J,Shen S,Shao S,Zhang C,Zhu Z,Dang E,Wang G

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Immunohistochemistry

Sat Oct 03 00:00:00 UTC 2020

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Cell death & disease

Zhang T,Yang L,Ke Y,Lei J,Shen S,Shao S,Zhang C,Zhu Z,Dang E,Wang G

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Immunohistochemistry

Sat Oct 03 00:00:00 UTC 2020

Targeting NEK2 attenuates glioblastoma growth and radioresistance by destabilizing histone methyltransferase EZH2.

The Journal of clinical investigation

Wang J,Cheng P,Pavlyukov MS,Yu H,Zhang Z,Kim SH,Minata M,Mohyeldin A,Xie W,Chen D,Goidts V,Frett B,Hu W,Li H,Shin YJ,Lee Y,Nam DH,Kornblum HI,Wang M,Nakano I

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Western Blot

Tue Aug 01 00:00:00 UTC 2017

Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes.

PLoS genetics

Wongtrakoongate P,Riddick G,Fucharoen S,Felsenfeld G

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Western Blot

Thu Oct 01 00:00:00 UTC 2015

Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes.

PLoS genetics

Wongtrakoongate P,Riddick G,Fucharoen S,Felsenfeld G

Published figure using EZH2 monoclonal antibody (Product # MA5-15101) in Western Blot

Thu Oct 01 00:00:00 UTC 2015

生物信息学

蛋白别名: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780

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基因别名: ENX-1; ENX1; Enx1h; EZH1; EZH2; EZH2b; KMT6; KMT6A; mKIAA4065; WVS; WVS2

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UniProt ID: (Human) Q15910, (Mouse) Q61188

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Entrez Gene ID: (Human) 2146, (Rat) 312299, (Mouse) 14056

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功能