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Rockland
Eg5 Polyclonal Antibody
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Eg5 Antibody (100-401-J04) in WB
Western Blot of Rabbit Anti-Eg-5 antibody. Lane 1: E.coli cell lysate expressing histidine tagged protein. Lane 2: Mouse brain lysate. Lane 3: Recombinant truncated Eg-5. Lane 4: MW Markers. Load: 35µg/lane for cell lysate, 50ng of recombinant protein. Primary antibody: Eg-5 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 120 kDa and 72 kDa for Eg5.
产品信息
100-401-J04
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Eg5 was prepared from whole rabbit serum produced by repeated immunizations with a truncated Eg5 construct expressed in E. coli corresponding to human Eg5 protein.
偶联物
形式
Liquid
浓度
46 mg/mL
保存液
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Eg5 is directed against the human Eg5 protein. A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with Eg5 from other sources have not been determined.
靶标信息
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Kines; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5
基因别名: EG5; HKSP; KIF11; KNSL1; MCLMR; TRIP5
UniProt ID: (Human) P52732
Entrez Gene ID: (Human) 3832
retrograde vesicle-mediated transport, Golgi to ER
microtubule-based movement
spindle organization
mitotic spindle organization
chromosome segregation
mitotic nuclear division
mitotic centrosome separation
antigen processing and presentation of exogenous peptide antigen via MHC class II
regulation of mitotic centrosome separation
cell division
mitotic spindle assembly
