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Invitrogen
Embigin Polyclonal Antibody
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Embigin Antibody (PA5-87887) in WB
Western blot analysis of Embigin in extracts of various cell lines. Samples were incubated with Embigin Polyclonal antibody (Product # PA5-87887) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-87887
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 33-260 of human EMB (NP_9408511)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.13 mg/mL
规格
113 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: DGSAPDSPFT SPPLREEIMA NNFSLESHNI SLTEHSSMPV EKNITLERPS NVNLTCQFTT SGDLNAVNVT WKKDGEQLEN NYLVSATGST LYTQYRFTII NSKQMGSYSC FFREEKEQRG TFNFKVPELH GKNKPLISYV GDSTVLTCKC QNCFPLNWTW YSSNGSVKVP VGVQMNKYVI NGTYANETKL KITQLLEEDG ESYWCRALFQ LGESEEHIEL VVLSYLVP; Positive Samples: Jurkat, MCF-7, THP-1; Cellular Location: Cell junction, Cell membrane, Single-pass type I membrane protein, synapse
靶标信息
Embigin is a 327 amino acid regulatory protein belonging to the immunoglobulin superfamily class of CAMs, and has two Ig-like (immunoglobulin-like) V- type domains. In situ hybridization analysis shows that Embigin has specific carbohydrate epitopes and plays an important role in early postimplantation embryogenesis. A highly glycosylated transmembrane glycoprotein, Embigin acts as a regulator of cell/ECM interactions during development and homeostasis of normal adult tissues. Transfection studies suggest its role in promotion of integrin-mediated cell-substratum adhesion. Widely expressed in most of the tissues and embryonal carcinoma cells, prostate and mammary cancer cell lines, it may also help in the classification of human carcinomas.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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