Invitrogen

Emerin Monoclonal Antibody (MANEM1(5D10))

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他17个Emerin抗体

说明书

实验方案

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说明书

实验方案

技术支持

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Emerin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Emerin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Emerin Monoclonal Antibody (MANEM1(5D10)) (Product # MA5-18076) at 1:25 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear membrane and endoplasmic reticulum localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Emerin Antibody in Western Blot (WB) — Western blot was performed using Anti-Emerin Monoclonal Antibody (MANEM1(5D10) (Product # MA5-18076) and a 32 kDa band corresponding to EMD was observed across cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), U-87MG (Lane 3), A-431 (Lane 4) and MCF-7 (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Emerin Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with EMD siRNA and a decrease in signal intensity was observed in western blot application using Anti-Emerin Monoclonal Antibody (MANEM1(5D10)) (Product # MA5-18076). {KD}

产品信息

MA5-18076

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:400-1:800	-
免疫细胞化学 (ICC/IF)	1:4-1:25	-

产品规格
种属反应	Human, Rabbit
宿主/亚型	Mouse / IgG1
分类	Monoclonal
类型	Antibody
克隆号	MANEM1(5D10)
抗原	Recombinant Human Emerin aa 1-188 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Protein G
保存液	PBS
内含物	0.1% sodium azide
保存条件	Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRID	AB_2539459

产品详细信息

This antibody does not cross-react with mouse samples.

This target has a predicted molecular weight of 29 kDa.

靶标信息

Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is an enzyme essential for DNA repair of the major kinds of solar ultraviolet (UV)-induced DNA damages. Human XPG nuclease makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981-1009) contains the PCNA binding activity. A conserved arginine in XPG (Arg992) is crucial for its PCNA binding activity. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, the xeroderma pigmentosum damage-recognition protein XPA and the endonuclease XPG.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Emerin; LEM domain containing 5

基因别名: EDMD; EMD; LEMD5; STA

UniProt ID: (Human) P50402

Entrez Gene ID: (Human) 2010, (Rabbit) 100328755

功能

muscle contraction mitotic nuclear envelope disassembly mitotic nuclear envelope reassembly muscle organ development negative regulation of catenin import into nucleus skeletal muscle cell differentiation positive regulation of protein export from nucleus negative regulation of fibroblast proliferation regulation of canonical Wnt signaling pathway cellular response to growth factor stimulus cell-cell adhesion

参与通路

actin binding protein binding beta-tubulin binding cadherin binding involved in cell-cell adhesion

Emerin Monoclonal Antibody (MANEM1(5D10))

产品信息

MA5-18076

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

种属反应

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

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