Proteintech

Endoglin/CD105 Polyclonal Antibody

23 篇参考文献

查看其他88个CD105 (Endoglin)抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (5)

Endoglin/CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Endoglin/CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 5

Endoglin/CD105 Antibody (10862-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Endoglin/CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Endoglin/CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Endoglin/CD105 Antibody in Western Blot (WB)

Endoglin/CD105 Antibody in Western Blot (WB)

Endoglin/CD105 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Endoglin/CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Endoglin/CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Endoglin/CD105 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Endoglin/CD105 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Endoglin/CD105 Antibody in Immunoprecipitation (IP) — IP result of anti-Endoglin/CD105 (IP:10862-1-AP, 3ug; Detection:10862-1-AP 1:300) with mouse lung tissue lysate 4000ug.

Immunogen image

Endoglin/CD105 Polyclonal Antibody

Immunogen Image Endoglin/CD105 Polyclonal Antibody

产品信息

10862-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:1,000

查看9篇已发表文章 9篇已发表文章

免疫组化 (IHC)

-

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

1:2,000-1:8,000

-

免疫细胞化学 (ICC/IF)

-

查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

-

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

0.5-4.0 µg

-

不适用 (N/A)

-

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Bovine, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Endoglin/CD105 Fusion Protein Ag1287 (331-658 aa encoded by BC014271)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: GGRLQTSPA PIQTTPPKDT CSPELLMSLI QTKCADDAMT LVLKKELVAH LKCTITGLTF WDPSCEAEDR GDKFVLRSAY SSCGMQVSAS MISNEAVVNI LSSSSPQRKK VHCLNMDSLS FQLGLYLSPH FLQASNTIEP GQQSFVQVRV SPSVSEFLLQ LDSCHLDLGP EGGTVELIQG RAAKGNCVSL LSPSPEGDPR FSFLLHFYTV PIPKTGTLSC TVALRPKTGS QDQEVHRTVF MRLNIISPDL SGCTSKGLVL PAVLGITFGA FLIGALLTAA LWYIYSHTRS PSKREPVVAV AAPASSESSS TNHSIGSTQS TPCSTSSMA (331-658 aa encoded by BC014271)

靶标信息

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CD105; CD105 antigen; Cell surface MJ7/18 antigen; Endoglin; ORW; transmembrane glycoprotein

查看更多收起

基因别名: AI528660; AI662476; CD105; Edg; END; Endo; ENG; HHT1; ORW1; S-endoglin

查看更多收起

UniProt ID: (Human) P17813, (Mouse) Q63961

查看更多收起

Entrez Gene ID: (Human) 2022, (Mouse) 13805, (Rat) 497010

查看更多收起

transmembrane signaling receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity activin binding transforming growth factor beta binding transforming growth factor beta-activated receptor activity transmembrane signal receptor

chronological cell aging patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation heart looping sprouting angiogenesis endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated cell adhesion negative regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis negative regulation of cell migration BMP signaling pathway positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation dorsal aorta morphogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis endocardial cushion to mesenchymal transition positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation negative regulation of transcription from RNA polymerase II promoter angiogenesis response to hypoxia negative regulation of endothelial cell proliferation positive regulation of systemic arterial blood pressure protein phosphorylation transmembrane receptor protein serine/threonine kinase signaling pathway transforming growth factor beta receptor signaling pathway heart development positive regulation of gene expression cell migration extracellular matrix disassembly positive regulation of collagen biosynthetic process intracellular signal transduction wound healing cell motility negative regulation of nitric-oxide synthase activity cell chemotaxis negative regulation of pathway-restricted SMAD protein phosphorylation extracellular matrix constituent secretion detection of hypoxia response to corticosteroid response to statin bone development cellular response to mechanical stimulus response to transforming growth factor beta