Invitrogen

Ephrin B (Pan) Monoclonal Antibody (2D3E9)

Name: Ephrin B (Pan) Monoclonal Antibody (2D3E9)
Brand: Invitrogen
SKU: 37-8100

6个已发表图片

6 篇参考文献

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (6)

Ephrin B (Pan) Antibody in Immunocytochemistry (ICC/IF)

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Ephrin B (Pan) Antibody (37-8100) in ICC/IF

Immunofluorescent analysis of Ephrin-B Pan was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ephrin-B Pan (2D3E9) Mouse Monoclonal Antibody (Product # 37-8100) at 2 µg/mL in 0.1% BSA and incubated for ... View More

Ephrin B (Pan) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Ephrin B (Pan) Antibody in Immunohistochemistry (IHC)

Ephrin B (Pan) Antibody in Western Blot, Immunoprecipitation (WB, IP)

Ephrin B (Pan) Antibody in Western Blot (WB)

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产品信息

37-8100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

2 µg/mL

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酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Chicken, Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2D3E9

抗原

Synthetic peptide derived from the ephrin-B sequence.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533342

靶标信息

Ephrin B genes, including Ephrin B1, B2, and B3, encode membrane-bound ligands that interact with Eph receptors, particularly EphB1 through EphB6. These ligands are crucial for various developmental processes, notably in the cardiovascular and nervous systems. Ephrin B2 is prominently expressed in arterial endothelial cells, while its receptor EphB4 is found in venous endothelial cells, highlighting a key distinction early in vascular development. Disruption of these genes can lead to significant vascular remodeling failures during embryogenesis. Ephrin-Eph receptor signaling involves the binding of ephrin molecules to their receptors on adjacent cells, initiating heterodimerization and receptor autophosphorylation, followed by recruitment of signaling clusters. This signaling is essential for various physiological and pathological functions, including heart development and function, as well as the formation and maintenance of the glomerular tuft in kidneys.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CEK5 ligand; CEK5-L; EFL-3; ELK ligand; ELK-L; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 2; EPH-related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 8; EphB receptor ligand; Ephrin-B1; Ephrin-B2; Ephrin-B3; HTK ligand; HTK-L; LERK-2; LERK-5; LERK-8; ligand of eph-related kinase 5; transmembrane ligand

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基因别名: CFND; CFNS; EFB1; EFL3; EFL6; EFNB1; EFNB2; EFNB3; Elk-L; EPLG2; EPLG5; EPLG8; Htk-L; HTKL; LERK2; LERK5; LERK8