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Invitrogen
ErbB2 (HER-2) Polyclonal Antibody
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ErbB2 (HER-2) Antibody (PA5-32343) in ICC/IF
Immunofluorescence analysis of Her2 was performed using 70% confluent log phase SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Her2 Rabbit polyclonal Antibody (Product # PA5-32343) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 de... View More
产品信息
PA5-32343
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide derived from the region of human c-erbB2 that contains non-phosphorylated tyrosine 1248
偶联物
形式
Liquid
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Gel ice packs
RRID
产品详细信息
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
靶标信息
ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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