Invitrogen

ErbB4 (Her4) Monoclonal Antibody (HFR1), eBioscience™

Product Image Gallery

ErbB4 (Her4) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma using 10 µg/mL Mouse IgG2b K Isotype Control Purified (left) or 10 µg/mL Anti-Human/Mouse ErbB4 (Her4) Purified (right) followed by Anti-Mouse IgG Biotin and DAB visualization.Nuclei are counterstained with hematoxylin.

产品信息

14-9687-80

应用	建议稀释比	已发表文章
免疫印迹 (WB)	Assay-Dependent	-
免疫组化（石蜡） (IHC (P))	10 µg/mL	-
免疫细胞化学 (ICC/IF)	10 µg/mL	-
免疫沉淀 (IP)	Assay-Dependent	-

产品规格
种属反应	Human, Mouse
宿主/亚型	Mouse / IgG2b, kappa
分类	Monoclonal
类型	Antibody
克隆号	HFR1
偶联物	Unconjugated Biotin
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	4° C
RRID	AB_2572955

产品详细信息

Description: The monoclonal antibody HFR1 recognizes human and mouse epidermal growth factor receptor 4 (ErbB4 or HER4). ErbB4 belongs to the family of receptor tyrosine kinases (RTK) of which HER1 (EGRF), HER2 (ErbB2), and HER3 (ErbB3) are members. RTKs function to affect a variety of cell processes including proliferation, differentiation, migration, survival and apoptosis and are often disregulated in cancer. The ErbB4 protein contains extracellular ligand binding domains, a transmembrane region, and a cytoplasmic tail containing the tyrosine kinase domain and the binding site for the HFR1 antibody. Upon ligand binding, ErbB4 homo- or hetero-dimerizes with other family members, becomes phosphorylated, and can be cleaved allowing translocation to the nucleus. The HRF1 antibody recognizes membrane-bound, cytoplasmic and nuclear ErbB4. Cytosolic ErbB4 expression was found to correlate with a positive prognosis in a subset of breast cancer patients, while nuclear ErbB4 expression was inversely correlated with tumor grade and aggressiveness of breast tumors.

Applications Reported: This HFR1 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This HFR1 antibody has been tested by immunocytochemistry on fixed and permeablilized A431 cells and can be used at less than or equal to 10 µg/mL. The HFR1 antibody has also been tested by immunohistochemistry on human FFPE tissue with low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ErbB 4 (Her-4, c-erbB-4, v-erbB-4) is a member of the class I receptor kinase family. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 4ICD; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; ERBB4 intracellular domain; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; s80HER4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

基因别名: ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4

UniProt ID: (Human) Q15303, (Mouse) Q61527

Entrez Gene ID: (Human) 2066, (Mouse) 13869

分子生物学功能: transmembrane signal receptor