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Invitrogen
Estrogen Receptor alpha Monoclonal Antibody (AER314)
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Estrogen Receptor alpha Antibody (MA5-12328) in WB
Western blot analysis of Estrogen Receptor (ER) was performed by loading the indicated amounts of recombinant full-length human ER alpha (Product # A15674) and ER beta (Product # A15664) proteins, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Blotter ... View More
产品信息
MA5-12328
应用
建议稀释比
已发表文章
产品规格
已发表种属
Bovine,
Hamster,
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, lambda
分类
Monoclonal
类型
Antibody
克隆号
AER314
抗原
Purified, SDS-denatured calf uterus estrogen receptor (ER)
偶联物
形式
Liquid
浓度
0.2 mg/mL
保存条件
4° C
运输条件
Wet ice
RRID
AB_10984048
产品详细信息
MA5-12328 targets Estrogen Receptor in WB, GS, and IP applications and shows reactivity with bovine, human, mouse, rabbit, and rat samples. By Western blot, MA5-12328 detects recombinant ER alpha, but may not be suitable for detection of endogenous protein.
The MA5-12328 immunogen is purified, SDS-denatured calf uterus estrogen receptor (ER).
靶标信息
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
