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Invitrogen
Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11)
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Estrogen Receptor alpha Antibody (MA5-13065) in WB
Western blot analysis of Estrogen Receptor (ER) was performed by loading the indicated amounts of recombinant full-length human ER alpha (Product # A15674) and ER beta (Product # A15664) proteins, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Blotter ... View More
产品信息
MA5-13065
应用
建议稀释比
已发表文章
产品规格
已发表种属
Bovine,
Chicken,
Human,
Mouse,
Pig,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
TE111.5D11
抗原
35kDa, C-terminal fragment (aa 302-595) of human ER expressed in E. coli
偶联物
形式
Liquid
浓度
0.2 mg/mL
保存条件
4°C
运输条件
Wet ice
RRID
产品详细信息
MA5-13065 targets Estrogen Receptor in ChIP, GS, IP, and WB applications and shows reactivity with Bovine, Canine, Chicken, Hamster, Human, mouse, Ovine, Porcine, Rabbit, and Rat samples. The TE111.5D11 clone was developed against the entire ER ligand binding domain (LBD), but has greatest affinity for ER-alpha. Because ER-alpha and ER-beta share 50-60% homology, MA5-13065 recognizes ER-beta1 with lower affinity.
The MA5-13065 immunogen is 35kDa, C-terminal fragment (aa 302-595) of human ER expressed in E. coli.
靶标信息
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
