Invitrogen

FASN Polyclonal Antibody

Name: FASN Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79238
Availability: InStock

高级验证

查看其他32个FASN抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (10)
高级验证 (1)

FASN Antibody in Immunocytochemistry (ICC/IF)

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FASN Antibody (PA5-79238) in ICC/IF

Immunofluorescence analysis of FASN was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FASN Polyclonal Antibody (Product # PA5-79238) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

FASN Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in HepG2 Cas9 cell line transduced with FASN Lentiviral sgRNA compared to control cell line using Anti-FASN Polyclonal Antibody (Product # PA5-79238). {KO}

Immunogen image

Immunogen Image FASN Polyclonal Antibody

产品信息

PA5-79238

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human FASN recombinant protein (Position: M1-E226).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746354

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human hepatocellular carcinoma tumor tissue (HCCT), human 293T whole cell, human MCF-7 whole cell, human RT4 whole cell, human A549 whole cell, rat liver tissue, mouse lung tissue, mouse liver tissue, mouse NIH/3T3 whole cell. IHC: human intestinal cancer tissue. ICC/IF: T47D cell.

靶标信息

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

仅用于科研。不用于诊断过程。未经明确授权不得转售。