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Invitrogen
FBXO33 Polyclonal Antibody
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FBXO33 Antibody (PA5-89740) in WB
Western blot analysis of FBXO33 in extracts of various cell lines. Samples were incubated with FBXO33 Polyclonal antibody (Product # PA5-89740) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
产品信息
PA5-89740
产品规格
种属反应
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 346-555 of human FBXO33 (NP_9760461)
偶联物
形式
Liquid
浓度
1.52 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: VMHKSLDNMP NDEHWKALSR KSTSFRVYIM AFDIKSEDML KILKPSIPLE RIHFDSYITC VSGAIVDLIS RQYDKFLTHF ILMNDVIDTS GFPDLSDNRN EDPLVLLAWR CTKLSLLAIH GYTVWAHNLI AIARLRGSDL KVLEVTEESI DFDQGELADQ DVDPVHNLIE QVSLGLGQPW HAVMDIESLS VFTEPNRHFY REMQSFSEDI; Positive Samples: Rat brain, Rat testis
靶标信息
FBXO33 is the substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO33 probably recognizes and binds to phosphorylated target proteins. Recognizes YBX1.Members of the F-box protein family, such as FBXO33, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: F-box only protein 33
Entrez Gene ID: (Rat) 314157
