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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • FOXJ1 Antibodies

          Invitrogen

          FOXJ1 Monoclonal Antibody (2A5), eBioscience™

          高级验证
          74个已发表图片
          119 篇参考文献
          查看其他14个FOXJ1抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite FOXJ1 Monoclonal Antibody (2A5), eBioscience™

          • 抗体检测数据 (2)
          • 已发表图片 (74)
          • 高级验证 (1)
          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          图: 1 / 77

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          FOXJ1 Antibody (14-9965-82) in IHC (P)

          Immunohistochemistry of paraffin-embedded mouse lung tissue with Anti-Human/Mouse FOXJ1 Purified followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Avidin HRP (Product # 18-4100-51) (right).Data provided courtesy of Steven L.Brody. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC, IHC (PFA))
          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          FOXJ1 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody in Western Blot, ChIP Assay (WB, ChIP)
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody

          产品信息

          14-9965-82

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1-10 µg/mL
          查看6篇已发表文章 6篇已发表文章

          免疫组化 (IHC)

          -
          查看62篇已发表文章 62篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1-10 µg/mL
          查看6篇已发表文章 6篇已发表文章

          免疫组织化学(多聚甲醛固定) (IHC (PFA))

          -
          查看3篇已发表文章 3篇已发表文章

          免疫组化(冰冻) (IHC (F))

          -
          查看5篇已发表文章 5篇已发表文章

          免疫细胞化学 (ICC/IF)

          Assay-Dependent
          查看33篇已发表文章 33篇已发表文章

          流式细胞分析 (Flow)

          -
          查看2篇已发表文章 2篇已发表文章

          染色质免疫沉淀 实验 (ChIP)

          -
          查看2篇已发表文章 2篇已发表文章
          产品规格

          种属反应

          Human, Mouse

          已发表种属

          Fruit fly, Human, Mouse, Pig, Rat

          宿主/亚型

          Mouse / IgG1

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          2A5

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1548835

          产品详细信息

          Description: This 2A5 monoclonal antibody reacts with Foxj1, a 50-kDa member of the Forkhead-box (FOX) family of winged-helix transcription factors. Expression of Foxj1 is high in ciliated epithelial cells and low in naive T and B cells, where cellular activation results in downregulation of the factor. Foxj1 modulates germinal center formation and Th1 activation by repressing NFkappaB through IkappaBbeta induction. As such, this transcription factor has been suggested to antagonize autoimmune reactions such as systemic lupus erythematosus and rheumatoid arthritis. In addition, Foxj1 has been linked to the formation of motile cilia in epithelial cells.

          Applications Reported: This 2A5 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

          Applications Tested: This 2A5 antibody has been tested by western blot of mouse tracheal epithelial cells and testes, as well as by immunohistochemistry of mouse lung tissue. This antibody can be used between 1-10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          Forkhead-box J1 (FOXJ1) is a 421-amino acid transcription factor that suppresses T cell activity and spontaneous autoimmunity, through the repression of NF B activity. FOXJ1 also inhibits the humoral immune response in B cells. FOXJ1 deficiency in B cells results in spontaneous and accentuated germinal center formation and is implicated in the development of pathogenic autoantibodies and accentuated responses to immunizations. Abnormal expression of FOXJ1 may be associated with autoimmune diseases and/or other inflammatory diseases. FOXJ1 is also required for cilia formation and left-right axis determination because it increases calpastatin expression, a protein necessary for the ability of basal bodies to anchor to the apical cytoskeleton. FOXJ1 expression may function as an early marker of epithelial cell differentiation, recovery, and function.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: fork head homologue 4; Forkhead box protein J1; forkhead transcription factor HFH-4; forkhead-like 13; Forkhead-related protein FKHL13; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; HNF-3/forkhead homolog 4; MGC35202

          查看更多 收起

          基因别名: FKHL-13; FKHL13; FOXJ1; HFH-4; HFH4

          查看更多 收起

          UniProt ID: (Human) Q92949, (Mouse) Q61660

          查看更多 收起

          Entrez Gene ID: (Human) 2302, (Mouse) 15223

          查看更多 收起

          功能
          transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding
          参与通路
          negative regulation of transcription from RNA polymerase II promoter central tolerance induction negative regulation of germinal center formation positive regulation of central B cell tolerance induction negative regulation of humoral immune response mediated by circulating immunoglobulin transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter humoral immune response cell projection organization actin cytoskeleton organization cell differentiation regulation of epithelial cell differentiation negative regulation of NF-kappaB transcription factor activity negative regulation of T cell differentiation in thymus establishment of apical/basal cell polarity negative regulation of T cell proliferation cilium assembly negative regulation of interleukin-6 biosynthetic process positive regulation of transcription from RNA polymerase II promoter negative regulation of B cell activation leukocyte migration cilium morphogenesis left/right pattern formation activation of GTPase activity positive regulation of lung ciliated cell differentiation spermatogenesis determination of left/right symmetry pattern specification process brain development heart development metanephric part of ureteric bud development lung epithelium development epithelium development glomerular parietal epithelial cell development
          It has to be done as per old AB suggested Products section.
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