Invitrogen

FOXO6 Polyclonal Antibody

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2 篇参考文献

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问题与答案

问题与答案

问题与答案

FOXO6 Antibody in Immunocytochemistry (ICC/IF)

FOXO6 Antibody in Immunocytochemistry (ICC/IF)

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FOXO6 Antibody (PA5-35117) in ICC/IF

Immunocytochemistry analysis of FOXO6 in U251 cells. Samples were incubated with FOXO6 polyclonal antibody (Product # PA5-35117) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). FOXO6... View More

FOXO6 Antibody in Immunocytochemistry (ICC/IF)

FOXO6 Antibody in Western Blot (WB)

FOXO6 Antibody in Western Blot, ChIP Assay (WB, ChIP)

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FOXO6 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of FOXO6 in U251 cells. Samples were incubated with FOXO6 polyclonal antibody (Product # PA5-35117) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). FOXO6 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

FOXO6 Antibody in Western Blot (WB) — Western blot analysis of FOXO6 in mouse heart tissue lysates. Samples were incubated with FOXO6 polyclonal antibody (Product # PA5-35117). Lysates: 35 µg/lane. FOXO6 protein (arrow).

FOXO6 Antibody in Western Blot, ChIP Assay (WB, ChIP) — Published figure using FOXO6 polyclonal antibody (Product # PA5-35117) in Western Blot and ChIP Assay

FOXO6 Antibody — RTKi induce IDH1 expression through FoxO6 (A) qRT-PCR of IDH1 in GIC-387 +/- E. (B-C) Western blot of IDH1 in GIC-387, GIC-20, and LN382 cells +/- TMZ or RTKi (representative of 2 independent experiments). (D) Gene organization of the 3 IDH1 isoforms with the position of an intronic FoxO6 binding site. (E) ChIP using FoxO6 antibody, followed by qRT-PCR of IDH1 (representative of 3 independent experiments). (F) Western blot for IDH1, effector caspases, FoxO6, and total and phosphorylated Akt in LN382 cells treated with siFoxO6, +/- RTKi (representative of 3 independent experiments). Veh, vehicle; E, erlotinib; LS, large subunit; LS+N, large subunit plus N-peptide; S+I, SU11274 + Imatinib; TMZ, temozolomide. See also Figure S6. {KD}

Immunogen image

FOXO6 Polyclonal Antibody

Immunogen Image FOXO6 Polyclonal Antibody

产品信息

PA5-35117

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

1:10-1:50

-

染色质免疫沉淀实验 (ChIP)

-

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产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 154-181 amino acids from the central region of human FOXO6

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

FOXO6 is a transcriptional activator.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Forkhead box protein O6

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基因别名: Foxo6

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UniProt ID: (Mouse) Q70KY4

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Entrez Gene ID: (Human) 100132074, (Mouse) 329934

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RNA polymerase II transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding

transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of dendritic spine development regulation of transcription, DNA-templated transcription from RNA polymerase II promoter anatomical structure morphogenesis cell differentiation