Invitrogen

FOXP3 Monoclonal Antibody (150D/E4), Alexa Fluor™ 488, eBioscience™

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FOXP3 Antibody (53-4774-42) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD25 APC (Product # 17-0251-82) and Anti-Mouse CD4 PE (Product # 12-0041-82) followed by fixation and permeabilization using Foxp3 Staining Buffers (Product # 00-5523-00) and subsequently stained with Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or Anti-Foxp3 Alexa Fluor® 488 (right). CD4+ cells in the lymphocyte gate were used for analysis.

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产品信息

53-4774-42

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

5 µL (0.5 µg)/test

查看8篇已发表文章 8篇已发表文章

染色质免疫沉淀实验 (ChIP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

150D/E4

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2043860

产品详细信息

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human/primate and mouse/rat Foxp3 protein, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to Foxp3 available from eBioscience, which have been used significantly in scientific literature, include the anti-human Foxp3 PCH101 (Product # 72-5776) and ebio7979 (Product # 12-7979), and the anti-mouse/rat Foxp3 FJK-16s (Product # 72-5775). The 150D/E4 has been mapped to the splice region in Exon 2 found in human cells. The splicing of this region has not been shown to occur in mouse. Characterization of this antibody to the splice variant has been limited to epitope mapping. It should be noted that other antibodies such as PCH101 and 236A/E7 will recognize both the spliced and full length forms of the Foxp3 protein and stain more intensely than 150D/E4 and FJK-16s in human cells.

The 150D/E4 antibody reacts with mouse/rat/human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of FoxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Applications Reported: This 150D/E4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 150D/E4 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The staining protocol has been optimized with freshly Ficoll-prepared PBMC or mouse splenocytes. The use of lysed whole blood is not recommended.

It is critical that this antibody be used in conjunction with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) for flow cytometric analysis.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

In mouse chronic pancreatitis CD25⁺FOXP3⁺ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response.

Nature communications

Glaubitz J,Wilden A,Golchert J,Homuth G,Völker U,Bröker BM,Thiele T,Lerch MM,Mayerle J,Aghdassi AA,Weiss FU,Sendler M

53-4774-42 was used in Immunocytochemistry-immunoflourescence to identify Treg cells as key regulators of the type-2 immune response and of organ remodeling during CP.

Wed Aug 03 00:00:00 EDT 2022

Retinoic Acid Induces Functionally Suppressive Foxp3⁺RORγt⁺ T Cells In Vitro.

Frontiers in immunology

Martínez-Blanco M,Lozano-Ojalvo D,Pérez-Rodríguez L,Benedé S,Molina E,López-Fandiño R

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in Flow Cytometry

Fri Jul 17 00:00:00 EDT 2020

Mitochondrial transfer from MSCs to T cells induces Treg differentiation and restricts inflammatory response.

EMBO reports

Court AC,Le-Gatt A,Luz-Crawford P,Parra E,Aliaga-Tobar V,Bátiz LF,Contreras RA,Ortúzar MI,Kurte M,Elizondo-Vega R,Maracaja-Coutinho V,Pino-Lagos K,Figueroa FE,Khoury M

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in Flow Cytometry

Wed Feb 05 00:00:00 EST 2020

Differential expression of tissue-restricted antigens among mTEC is associated with distinct autoreactive T cell fates.

Nature communications

Lebel MÈ,Coutelier M,Galipeau M,Kleinman CL,Moon JJ,Melichar HJ

53-4774 was used in Flow cytometry/Cell sorting to investigate whether medullary thymic epithelial cells subsets induce distinct autoreactive T cell fates.

Fri Jul 24 00:00:00 EDT 2020

Differential expression of tissue-restricted antigens among mTEC is associated with distinct autoreactive T cell fates.

Nature communications

Lebel MÈ,Coutelier M,Galipeau M,Kleinman CL,Moon JJ,Melichar HJ

53-4774 was used in Flow cytometry/Cell sorting to investigate whether medullary thymic epithelial cells subsets induce distinct autoreactive T cell fates.

Fri Jul 24 00:00:00 EDT 2020

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Microbial, metabolomic, and immunologic dynamics in a relapsing genetic mouse model of colitis induced by T-synthase deficiency.

Gut microbes

Jacobs JP,Lin L,Goudarzi M,Ruegger P,McGovern DP,Fornace AJ,Borneman J,Xia L,Braun J

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in Flow Cytometry

Mon Jan 02 00:00:00 EST 2017

Microbial, metabolomic, and immunologic dynamics in a relapsing genetic mouse model of colitis induced by T-synthase deficiency.

Gut microbes

Jacobs JP,Lin L,Goudarzi M,Ruegger P,McGovern DP,Fornace AJ,Borneman J,Xia L,Braun J

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in Flow Cytometry

Mon Jan 02 00:00:00 EST 2017

Histone/protein deacetylase 11 targeting promotes Foxp3+ Treg function.

Scientific reports

Huang J,Wang L,Dahiya S,Beier UH,Han R,Samanta A,Bergman J,Sotomayor EM,Seto E,Kozikowski AP,Hancock WW

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in ChIP assay

Thu Aug 17 00:00:00 EDT 2017

Characteristics of CD4⁺CD25⁺Foxp3⁺ regulatory T cells in patients with multiple organ dysfunction syndrome.

Experimental and therapeutic medicine

Zang X,Jiang Y,Li X,DU Y,Niu J

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants.

Nature immunology

De Rosa V,Galgani M,Porcellini A,Colamatteo A,Santopaolo M,Zuchegna C,Romano A,De Simone S,Procaccini C,La Rocca C,Carrieri PB,Maniscalco GT,Salvetti M,Buscarinu MC,Franzese A,Mozzillo E,La Cava A,Matarese G

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in Western Blot

Sun Nov 01 00:00:00 EDT 2015

CD40 deficiency in mice exacerbates obesity-induced adipose tissue inflammation, hepatic steatosis, and insulin resistance.

American journal of physiology. Endocrinology and metabolism

Guo CA,Kogan S,Amano SU,Wang M,Dagdeviren S,Friedline RH,Aouadi M,Kim JK,Czech MP

Published figure using FOXP3 monoclonal antibody (Product # 53-4774-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

生物信息学

蛋白别名: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy

查看更多收起

基因别名: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; RGD1562112; scurfin; sf; XPID

查看更多收起

UniProt ID: (Human) Q9BZS1, (Mouse) Q99JB6

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Entrez Gene ID: (Human) 50943, (Mouse) 20371, (Rat) 317382

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功能

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding nucleic acid binding DNA binding molecular_function

参与通路

FOXP3 Monoclonal Antibody (150D/E4), Alexa Fluor™ 488, eBioscience™

FOXP3 Antibody (53-4774-42) in Flow

产品信息

53-4774-42

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!

FOXP3 Monoclonal Antibody (150D/E4), Alexa Fluor™ 488, eBioscience™

FOXP3 Antibody (53-4774-42) in Flow

产品信息

53-4774-42

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)