Invitrogen

FUBP1 Polyclonal Antibody

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FUBP1 Antibody (PA5-96364) in WB

Western blot analysis of FUBP1 in extracts of various cell lines. Samples were incubated with FUBP1 Polyclonal antibody (Product # PA5-96364) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Product Image Gallery

FUBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FUBP1 in paraffin-embedded human liver damage. Samples were incubated with FUBP1 Polyclonal antibody (Product # PA5-96364) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

FUBP1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of FUBP1 in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg FUBP1 Polyclonal antibody (Product # PA5-96364). Western blot was performed from the immunoprecipitate using FUBP1 Polyclonal antibody (Product # PA5-96364) at a dilution of 1:1,000.

FUBP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of FUBP1 in C6 cells. Samples were incubated with FUBP1 Polyclonal antibody (Product # PA5-96364) using a dilution of 1:100. Blue: DAPI for nuclear staining.

产品信息

PA5-96364

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:100

免疫沉淀 (IP)

1:50 - 1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 302-644 of human FUBP1.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.74 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2808166

产品详细信息

Immunogen sequence: QNDAGVRIQF KPDDGTTPER IAQITGPPDR CQHAAEIITD LLRSVQAGNP GGPGPGGRGR GRGQGNWNMG PPGGLQEFNF IVPTGKTGLI IGKGGETIKS ISQQSGARIE LQRNPPPNAD PNMKLFTIRG TPQQIDYARQ LIEEKIGGPV NPLGPPVPHG PHGVPGPHGP PGPPGPGTPM GPYNPAPYNP GPPGPAPHGP PAPYAPQGWG NAYPHWQQQA PPDPAKAGTD PNSAAWAAYY AHYYQQQAQP PPAAPAGAPT TTQTNGQGDQ QNPAPAGQVD YTKAWEEYYK KMGQAVPAPT GAPPGGQPDY SAAWAEYYRQ QAAYYAQTSP QGMPQHPPAP QGQ; Positive Samples: U-87MG, HeLa, HepG2, THP-1, Mouse brain; Cellular Location: Nucleus

靶标信息

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DNA helicase V; far upstream element (FUSE) binding protein 1; far upstream element (FUSE) binding protein 4; Far upstream element-binding protein 1; far upstream element-binding protein 1 {ECO:0000250|UniProtKB:Q96AE4, ECO:0000312|EMBL:AAI07943.1}; FBP; FBP {ECO:0000250|UniProtKB:Q96AE4}; fubp1 {ECO:0000312|EMBL:AAI07943.1, ECO:0000312|RGD:1591892}; FUSE binding protein 1; FUSE-binding protein 1; FUSE-binding protein 1 {ECO:0000250|UniProtKB:Q96AE4}; hDH V

基因别名: 9530027K12Rik; D3Ertd330e; FBP; FUBP; FUBP1; Fubp4; hDH V

UniProt ID: (Human) Q96AE4, (Rat) Q32PX7

Entrez Gene ID: (Human) 8880, (Rat) 654496, (Mouse) 51886

分子生物学功能: RNA metabolism protein