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Invitrogen
FXN Polyclonal Antibody
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FXN Antibody (PA5-95829) in ICC/IF
Immunofluorescence analysis of Frataxin, mitochondrial was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FXN Polyclonal Antibody (Product # PA5-95829) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product... View More
产品信息
PA5-95829
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN / Frataxin (NP_0001352)
偶联物
形式
Liquid
浓度
1.76 mg/mL
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: LRTDIDATCT PRRASSNQRG LNQIWNVKKQ SVYLMNLRKS GTLGHPGSLD ETTYERLAEE TLDSLAEFFE DLADKPYTFE DYDVSFGSGV LTVKLGGDLG TYVINKQTPN KQIWLSSPSS GPKRYDWTGK NWVYSHDGVS LHELLAAELT KALKTKLDLS SLAYSGKDA; Positive Samples: K-562, U-87MG, Jurkat, Raji, DU-145, Mouse kidney, Mouse liver, Mouse heart; Cellular Location: Cytoplasm, Mitochondrion
靶标信息
FXN is a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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