Invitrogen

FXR Monoclonal Antibody (A9033A)

3个已发表图片
9 篇参考文献
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说明书
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说明书
实验方案
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产品信息

417200

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1 µg/mL
查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

-
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免疫组化（冰冻） (IHC (F))

20-40 µg/mL
-

免疫细胞化学 (ICC/IF)

-
查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

0.2 µg/mL
-

免疫沉淀 (IP)

Assay-dependent
-
产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

A9033A

抗原

Baculovirus-expressed recombinant human FXR (2-126 aa).
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

physiological saline

内含物

0.1% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

运输条件

Wet ice

RRID

AB_2532196

产品详细信息

This antibody specifically recognizes human FXR and cross reacts with mouse and rat FXR.

靶标信息

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Bile acid receptor; farnesoid X activated receptor; farnesoid X nuclear receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14

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基因别名: AI957360; BAR; FXR; HRR-1; HRR1; NR1H4; RIP14; Rxrip14

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UniProt ID: (Human) Q96RI1, (Rat) Q62735, (Mouse) Q60643

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Entrez Gene ID: (Human) 9971, (Rat) 60351, (Mouse) 20186

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功能
RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity transcription coactivator activity transcription corepressor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding thyroid hormone receptor activity protein binding zinc ion binding ligand-dependent nuclear receptor binding bile acid binding bile acid receptor activity peptide binding sequence-specific DNA binding retinoid X receptor binding chenodeoxycholic acid binding RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA binding double-stranded DNA binding receptor activity metal ion binding
参与通路
negative regulation of transcription from RNA polymerase II promoter regulation of carbohydrate metabolic process regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter bile acid metabolic process response to glucose intracellular receptor signaling pathway response to lipopolysaccharide regulation of urea metabolic process intracellular bile acid receptor signaling pathway steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter digestive tract development negative regulation of bile acid biosynthetic process cellular response to organonitrogen compound cellular response to bile acid positive regulation of glutamate metabolic process positive regulation of ammonia assimilation cycle nitrogen catabolite activation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated Notch signaling pathway transcription initiation from RNA polymerase II promoter inflammatory response signal transduction regulation of low-density lipoprotein particle clearance bile acid and bile salt transport toll-like receptor 4 signaling pathway histone H3-R17 methylation cellular triglyceride homeostasis bile acid signaling pathway negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of insulin secretion involved in cellular response to glucose stimulus regulation of bile acid biosynthetic process cellular response to fatty acid interleukin-17 secretion regulation of cholesterol metabolic process negative regulation of interferon-gamma secretion negative regulation of tumor necrosis factor secretion
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