Invitrogen

FXR Monoclonal Antibody (A9033A)

FXR Antibody in Immunohistochemistry (Frozen) (IHC (F))

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FXR Antibody (417200) in IHC (F)

Immunohistochemistry analysis of FRX in frozen rat liver tissues. The sample was inclubated in FRX monoclonal antibody (Product # 417200) at a dilution of 3~20 μg/mL. Section was fixed with 4% paraformaldehyde-fixed in immersion, activated with 5% TritonX-100 for 5 minutes, and blocked with 3% H2O2 in methanol.

Product Image Gallery

FXR Antibody in Immunocytochemistry (ICC/IF) — Figure 1 ( a ) Immunolabelling of farnesoid X receptor, FXR (red) in rat cerebral cortex, hippocampus, and cerebellum of control and thioacetamide (TAA) rats. Neurons were positively immunostained against the neuronal marker NeuN (yellow). Astrocytes were identified by immunostaining against the glial fibrillary acidic protein (GFAP, green). Blue Hoechst stain indicates the location of cell nuclei. Dashed lines indicate the Purkinje layer. Scale bars = 10 mum; ( b ) immunolabelling of FXR in the primary cultures of rat cerebellar neurons. Neurons were positively immunostained against the neuronal marker neurofilament 200 (NF200, green). Blue Hoechst stain indicates the location of cell nuclei. Scale bars = 10 mum.

FXR Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 6 Detection of bile acid receptors in freshly isolated and activated HSC from rats. Freshly isolated (1 day) and cultured (4 days) HSC were stained with antibodies against the bile acid receptors ( a,b ) Fxr and ( c,d ) Tgr5 (red). The mesenchymal markers desmin and vimentin (green) were used to verify the presence of stellate cells. HSC cultured for 4 days were maintained in medium without serum and without TUDCA. ( a ) Few freshly isolated HSC displayed weak nuclear staining of Fxr, but most cells lacked clear nuclear Fxr staining one day after isolation. ( b ) The majority of HSC exhibited significant nuclear Fxr after few days in culture. ( c ) Tgr5 was not detectable in freshly isolated HSC by immunofluorescence, ( d ) but appeared during culture within few days. ( e ) The presence of Fxr in freshly isolated and cultured HSC was confirmed by Western blot analysis. Fxr was detected in the nuclear protein faction mainly after 4 days of culture (10 mug protein per lane). ( f ) Tgr5 was found in cell membrane protein fraction of HSC after 4 days of culture (30 mug protein per lane). The protein gamma-tubulin served as a control.

Product Details

417200

Applications

Tested Dilution

Publications

免疫印迹 (WB)

1 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

20-40 µg/mL

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

0.2 µg/mL

免疫沉淀 (IP)

Assay-dependent

产品规格

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

A9033A

Immunogen

Baculovirus-expressed recombinant human FXR (2-126 aa).

View immunogen

Conjugate

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

physiological saline

内含物

0.1% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

RRID

AB_2532196

Product Specific Information

This antibody specifically recognizes human FXR and cross reacts with mouse and rat FXR.

Target Information

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hyperoside attenuates non-alcoholic fatty liver disease in rats via cholesterol metabolism and bile acid metabolism.

Journal of advanced research

Wang S,Sheng F,Zou L,Xiao J,Li P

417200 was used in Western Blot to suggest that hyperoside could improve the condition of NAFLD by regulating the cholesterol metabolism as well as BAs metabolism and excretion.

Wed Dec 01 00:00:00 EST 2021

Pregnancy and weaning regulate human maternal liver size and function.

Proceedings of the National Academy of Sciences of the United States of America

Q Bartlett A,Vesco KK,Purnell JQ,Francisco M,Goddard E,Guan X,DeBarber A,Leo MC,Baetscher E,Rooney W,Naugler W,Guimaraes AR,Catalano P,Xia Z,Schedin P

417200 was used in Western Blot to support the hypothesis that the human liver is regulated by reproductive state with growth during pregnancy and volume loss postwean.

Tue Nov 30 00:00:00 EST 2021

FXR Isoforms Control Different Metabolic Functions in Liver Cells via Binding to Specific DNA Motifs.

Gastroenterology

Ramos Pittol JM,Milona A,Morris I,Willemsen ECL,van der Veen SW,Kalkhoven E,van Mil SWC

417200 was used in Western Blotting to discover a novel DNA-binding feature of FXR that explains isoform selectivity.

Sun Nov 01 00:00:00 EDT 2020

The Status of Bile Acids and Farnesoid X Receptor in Brain and Liver of Rats with Thioacetamide-Induced Acute Liver Failure.

International journal of molecular sciences

Czarnecka AM,Milewski K,Albrecht J,Zielińska M

Published figure using FXR monoclonal antibody (Product # 417200) in Immunocytochemistry

Tue Oct 20 00:00:00 EDT 2020

The Status of Bile Acids and Farnesoid X Receptor in Brain and Liver of Rats with Thioacetamide-Induced Acute Liver Failure.

International journal of molecular sciences

Czarnecka AM,Milewski K,Albrecht J,Zielińska M

Published figure using FXR monoclonal antibody (Product # 417200) in Immunocytochemistry

Tue Oct 20 00:00:00 EDT 2020

The Status of Bile Acids and Farnesoid X Receptor in Brain and Liver of Rats with Thioacetamide-Induced Acute Liver Failure.

International journal of molecular sciences

Czarnecka AM,Milewski K,Albrecht J,Zielińska M

Published figure using FXR monoclonal antibody (Product # 417200) in Immunocytochemistry

Tue Oct 20 00:00:00 EDT 2020

Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis.

Nutrition & metabolism

Li H,Xi Y,Xin X,Tian H,Hu Y

417200 was used in Western Blot to elucidate the mechanism of Gyp in non-alcoholic steatohepatitis (NASH) with a focus on farnesoid X receptor (FXR)-mediated bile acid and lipid metabolic pathways.

Thu Apr 14 00:00:00 EDT 2022

Bile acids induce hepatic differentiation of mesenchymal stem cells.

Scientific reports

Sawitza I,Kordes C,Götze S,Herebian D,Häussinger D

Published figure using FXR monoclonal antibody (Product # 417200) in Immunofluorescence

Tue Aug 25 00:00:00 EDT 2015

Bile acids induce hepatic differentiation of mesenchymal stem cells.

Scientific reports

Sawitza I,Kordes C,Götze S,Herebian D,Häussinger D

Published figure using FXR monoclonal antibody (Product # 417200) in Immunofluorescence

Tue Aug 25 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Bile acid receptor; farnesoid X activated receptor; farnesoid X nuclear receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14

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Gene Aliases: AI957360; BAR; FXR; HRR-1; HRR1; NR1H4; RIP14; Rxrip14

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UniProt ID: (Human) Q96RI1, (Rat) Q62735, (Mouse) Q60641

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Entrez Gene ID: (Human) 9971, (Rat) 60351, (Mouse) 20186

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Function(s)

negative regulation of transcription from RNA polymerase II promoter regulation of carbohydrate metabolic process regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter bile acid metabolic process response to glucose intracellular receptor signaling pathway response to lipopolysaccharide regulation of urea metabolic process intracellular bile acid receptor signaling pathway steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter digestive tract development negative regulation of bile acid biosynthetic process cellular response to organonitrogen compound cellular response to bile acid positive regulation of glutamate metabolic process positive regulation of ammonia assimilation cycle nitrogen catabolite activation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated Notch signaling pathway transcription initiation from RNA polymerase II promoter inflammatory response signal transduction regulation of low-density lipoprotein particle clearance bile acid and bile salt transport toll-like receptor 4 signaling pathway histone H3-R17 methylation cellular triglyceride homeostasis bile acid signaling pathway negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of insulin secretion involved in cellular response to glucose stimulus regulation of bile acid biosynthetic process cellular response to fatty acid interleukin-17 secretion regulation of cholesterol metabolic process negative regulation of interferon-gamma secretion negative regulation of tumor necrosis factor secretion

Process(es)

RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity transcription coactivator activity transcription corepressor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding thyroid hormone receptor activity protein binding zinc ion binding ligand-dependent nuclear receptor binding bile acid binding bile acid receptor activity peptide binding sequence-specific DNA binding retinoid X receptor binding chenodeoxycholic acid binding RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA binding double-stranded DNA binding receptor activity metal ion binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FXR Monoclonal Antibody (A9033A)

FXR Antibody (417200) in IHC (F)

Product Details

417200

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

Species Reactivity

Published species

Host/Isotype

分类

类型

克隆号

Immunogen

Conjugate

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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