Invitrogen

FZD8 Polyclonal Antibody

Name: FZD8 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-120635

查看其他11个FZD8抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

FZD8 Antibody (PA5-120635) in WB

Western blot analysis of FZD8 in extracts of various cell lines. Samples were incubated with FZD8 Polyclonal antibody (Product # PA5-120635) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Product Image Gallery

Immunogen image

Immunogen Image FZD8 Polyclonal Antibody

产品信息

PA5-120635

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 130-275 of human FZD8 (NP_114072.1).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.96 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2914207

产品详细信息

Positive test controls include: 293T, A-549, HeLa, Mouse liver, Rat liver.

Immunogen sequence: PDRMRCDRLP EQGNPDTLCM DYNRTDLTTA APSPPRRLPP PPPGEQPPSG SGHGRPPGAR PPHRGGGRGG GGGDAAAPPA RGGGGGGKAR PPGGGAAPCE PGCQCRAPMV SVSSERHPLY NRVKTGQIAN CALPCHNPFF SQDERA

靶标信息

Members of the Frizzled family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Frizzled 8 is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. Its expression has been reported in brain, heart, kidney, pancreas, and skeletal muscle. ESTs have been isolated from embryo, eye, head/neck, heart, kidney, liver/spleen, pancreas, prostate, and testis libraries, as well as many tumor libraries. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。