Rockland

Fbp5B Polyclonal Antibody

Name: Fbp5B Polyclonal Antibody
Brand: Rockland
SKU: 600-401-485

查看其他6个FBXO43抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

Fbp5B Antibody (600-401-485) in WB

Western blot using Rocklands affinity purified anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This pre-incubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band f... View More

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Immunogen image

Immunogen Image Fbp5B Polyclonal Antibody

产品信息

600-401-485

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500- 1:2,000

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000

免疫沉淀 (IP)

1:500-1:3,000

产品规格

种属反应

Chimpanzee, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein. Human Fbp5B-2, 466 aa, predicted MW 51.3 kDa

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.21 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity-purified antibody is directed against human Fbp5B protein. A BLAST analysis was used to suggest cross reactivity with Fbp5B protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

靶标信息

Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1., cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 ).

仅用于科研。不用于诊断过程。未经明确授权不得转售。