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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
流式细胞分析 (Flow) |
5 µL (10 µg)/test | - |
| 产品规格 | |
|---|---|
种属反应 |
Rhesus monkey |
宿主/亚型 |
Not Applicable |
分类 |
Polyclonal |
类型 |
Antibody |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
5 µL/Test |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2, with 0.2% BSA |
内含物 |
0.09% sodium azide |
保存条件 |
4° C |
RRID |
AB_2572937 |
Description: The Rhesus Fc Receptor Binding Inhibitor can be used to inhibit the non-specific Fc receptor (FcR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of rhesus tissue. FcR are expressed at varying levels in multiple cell lineages including high expression in myeloid cells, granulocytes, and B cells. The biological function of the FcR, including initiation of endocytosis, phagocytosis, and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to rhesus FcR varies depending on the isotype of the monoclonal antibody.
eBioscience testing indicates that mouse IgG2a isotype is blocked by Rhesus Fc Receptor Binding Inhibitor.
Applications Reported: Rhesus Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis.
To inhibit the non-specific binding of mouse monoclonal antibodies add the Rhesus Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing, proceed to stain with primary antibody.
Applications Tested: Rhesus Fc Receptor Binding Inhibitor has been pre-titrated and tested by flow cytometric analysis of normal rhesus peripheral blood cells. This can be used at 5 µL (10 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Fc blocking; FcR BI; FcR Inhibitor
