Invitrogen

G3BP1 Polyclonal Antibody

G3BP1 Antibody in Immunocytochemistry (ICC/IF)

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G3BP1 Antibody (PA5-90073) in ICC/IF

Immunofluorescence analysis of Ras GTPase-activating protein-binding protein 1 was performed using 70% confluent log phase HeLa cells treated with heat shock at 45 degree centigrade for 60 minutes. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with G3BP1 Polyclonal Antibody (Product # PA5-90073) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donk... View More

G3BP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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G3BP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Ras GTPase-activating protein-binding protein 1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-G3BP1 Polyclonal Antibody (Product # PA5-90073). {KD}

产品信息

PA5-90073

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

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免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫细胞化学 (ICC/IF)

1:50-1:100

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 131-330 of human G3BP1 (NP_9384051)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.75 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2805911

产品详细信息

Immunogen sequence: FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAPEDAQKS SSPAPADIAQ TVQEDLRTFS WASVTSKNLP PSGAVPVTGI PPHVVKVPAS QPRPESKPES QIPPQRPQRD QRVREQRINI PPQRGPRPIR EAGEQGDIEP; Positive Samples: A-431, HeLa, 293T, Jurka, MCF7; Cellular Location: Cell membrane, Cytoplasm, Cytoplasmic granule, Nucleus, cytosol

靶标信息

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Stress granule activation attenuates lipopolysaccharide-induced cardiomyocyte dysfunction.

BMC cardiovascular disorders

Wang Y,Liu R,Wu K,Yang G,Wang Y,Wang H,Rui T

PA5-90073 was used in Western Blotting to determine the effects of SG activation in septic cardiomyocytes (CMs).

Sat May 27 00:00:00 UTC 2023

生物信息学

蛋白别名: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP

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基因别名: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666

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UniProt ID: (Human) Q13283, (Mouse) P97855

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Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041

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功能