Invitrogen

G3BP2 Polyclonal Antibody

查看其他6个G3BP2抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (3)

G3BP2 Antibody in Immunocytochemistry (ICC/IF)

G3BP2 Antibody in Immunocytochemistry (ICC/IF)

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G3BP2 Antibody (PA5-101815) in ICC/IF

Immunocytochemistry analysis of G3BP2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with G3BP2 polyclonal antibody (Product # PA5-101815) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

G3BP2 Antibody in Immunocytochemistry (ICC/IF)

G3BP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

G3BP2 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

G3BP2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of G3BP2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with G3BP2 polyclonal antibody (Product # PA5-101815) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

G3BP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of G3BP2 in mouse liver tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with G3BP2 polyclonal antibody (Product # PA5-101815) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

G3BP2 Antibody in Western Blot (WB) — Western blot analysis of G3BP2 in extracts from various samples. Samples were incubated with G3BP2 polyclonal antibody (Product # PA5-101815). Lane 1: Mouse brain, blocked with antigen-specific peptides; Lane 2: Mouse brain; Lane 3: Hepg2 cells (heat-shock treatment); Lane 4: Hela cells (heat-shock treatment).

Immunogen image

G3BP2 Polyclonal Antibody

Immunogen Image G3BP2 Polyclonal Antibody

产品信息

PA5-101815

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human G3BP2(Accession Q9UN86), corresponding to amino acid residues D450-R482.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total G3BP2.

靶标信息

G3BP2 gene ontology annotations related to this gene include stress granule assembly.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: G3BP-2; GAP SH3 domain-binding protein 2; Ras GTPase-activating protein-binding protein 2

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基因别名: AA409541; E430034L04Rik; G3BP; G3BP2; KIAA0660; mKIAA0660; RGD1309571

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UniProt ID: (Mouse) P97379

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Entrez Gene ID: (Rat) 305240, (Mouse) 23881

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nucleotide binding nucleic acid binding poly(A) RNA binding RNA metabolism protein nucleic acid metabolism protein

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