Invitrogen

GAD67 Polyclonal Antibody

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GAD67 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of GAD67 in A431 cells. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with GAD67 polyclonal antibody (Product # PA5-94936) at a 3 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and developed with Strepavidin-Biotin-Complex/DAB.

GAD67 Antibody in Western Blot (WB) — Western blot was performed using Anti-GAD67 Polyclonal Antibody (Product # PA5-94936) and a 67kDa band corresponding to GAD67 was observed across all cell lines and tissues tested except Mouse Skeletal Muscle and Rat Liver which are known to be negative. Membrane enriched extracts (30 µg lysate) of Neuro-2a (Lane 1), Mouse Brain (Lane 2), Rat Brain (Lane 3), Mouse Skeletal Muscle (Lane 4) and Rat Liver (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5ug/ml dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

产品信息

PA5-94936

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫细胞化学 (ICC/IF)

0.5-1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human GAD67 (529-543aa PQRREKLHKVAPKIK).

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偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2806742

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 67 kDa glutamic acid decarboxylase; FLJ45882; GAD 67; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 variant GAD67NT; Glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); glutamic acid decarboxylase 1; OTTHUMP00000041055

基因别名: CPSQ1; EP10; GAD; Gad-1; GAD1; GAD25; GAD44; GAD67; SCP

UniProt ID: (Human) Q99259, (Mouse) P48318, (Rat) P18088

Entrez Gene ID: (Human) 2571, (Mouse) 14415, (Rat) 24379

分子生物学功能: decarboxylase