应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
0.2-2.5 µg/mL | - |
免疫组化(石蜡) (IHC (P)) |
2-10 µg/mL | - |
产品规格 | |
---|---|
种属反应 |
Human |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Synthetic peptide region of human GATA3. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
PBS with 2% sucrose |
内含物 |
0.09% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2108576 |
Recommended positive controls: Jurkat.
Predicted reactivity: Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Mouse: 93%; Rabbit: 93%; Rat: 93%; Sheep: 93%.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: GATA binding protein-3; GATA-binding factor 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
基因别名: GATA3; HDR; HDRS
UniProt ID: (Human) P23771
Entrez Gene ID: (Human) 2625
分子生物学功能:
DNA-binding transcription factor