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Invitrogen
GCN2 Recombinant Rabbit Monoclonal Antibody (JA03-83)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 11
GCN2 Antibody (MA5-32704) in ICC/IF
Immunofluorescence analysis of GCN2 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GCN2 Recombinant Rabbit Monoclonal Antibody (JA03-83) (Product # MA5-32704) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More
产品信息
MA5-32704
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JA03-83
抗原
Synthetic peptide within Human GCN2 aa 1-50
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809981
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
EIF2AK4 (GCN2) is a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor-2 in response to low amino acid availability. It plays a role as an activator of the integrated stress response required for adapatation to amino acid starvation. Mutations in the gene lead to pulmonary vennocclusive disease 2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eIF-2-alpha kinase GCN2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2 eIF2alpha kinase; GCN2-like protein; general control nonderepressible 2
基因别名: EIF2AK4; GCN2; KIAA1338; PVOD2
UniProt ID: (Human) Q9P2K8
Entrez Gene ID: (Human) 440275
positive regulation of defense response to virus by host
adaptive immune response
T cell activation involved in immune response
positive regulation of adaptive immune response
regulation of translational initiation
protein phosphorylation
cell cycle arrest
learning
long-term memory
regulation of translational initiation by eIF2 alpha phosphorylation
viral translation
negative regulation of translational initiation in response to stress
negative regulation of CREB transcription factor activity
cellular response to amino acid starvation
cellular response to UV
eiF2alpha phosphorylation in response to endoplasmic reticulum stress
induction by virus of host autophagy
negative regulation by host of viral genome replication
negative regulation of neuron differentiation
negative regulation of translational initiation
protein autophosphorylation
defense response to virus
regulation of feeding behavior
regulation of eIF2 alpha phosphorylation by amino acid starvation
cellular response to cold
positive regulation of translational initiation in response to starvation
positive regulation of long-term synaptic potentiation
neuron projection extension
cellular response to leucine starvation
