Invitrogen

GFAP Monoclonal Antibody (GA5), eFluor™ 570, eBioscience™

Name: GFAP Monoclonal Antibody (GA5), eFluor\u2122 570, eBioscience\u2122
Brand: Invitrogen
SKU: 41-9892-82

高级验证

34个已发表图片

33 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

GFAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GFAP Antibody (41-9892-82) in IHC (P)

Immunohistochemical analysis of GFAP was performed using formalin-fixed paraffin-embedded mouse brain and mouse ovary tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 1... View More

GFAP Antibody in Immunohistochemistry (IHC)

GFAP Antibody in Immunocytochemistry (ICC/IF)

GFAP Antibody in Immunohistochemistry (Frozen), Immunocytochemistry (IHC (F), ICC/IF)

GFAP Antibody in Immunohistochemistry (Frozen) (IHC (F))

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GFAP Antibody — Antibody specificity was demonstrated by the relative expression of target protein in different tissues by IHC. Differential expression of GFAP in mouse brain and mouse ovary tissues using GFAP Monoclonal Antibody (GA5), eFluor™ 570, eBioscience™ (Product # 41-9892-82) shows strong staining of GFAP in mouse brain tissue and no staining in mouse ovary tissue. {RE}

产品信息

41-9892-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1-4 µg/mL

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

Assay-Dependent

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产品规格

种属反应

Chicken, Human, Mouse, Pig, Rabbit, Rat

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

GA5

偶联物

eFluor™ 570 eFluor™ 570 eFluor™ 570

激发/发射光谱

556/569 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573656

产品详细信息

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.

Applications Reported: This GA5 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This GA5 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval. The antibody can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

靶标信息

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

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基因别名: AI836096; ALXDRD; GFAP

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UniProt ID: (Human) P14136, (Rat) P47819, (Mouse) P03995