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GLI3 Polyclonal Antibody

GLI3 Antibody in Immunocytochemistry (ICC/IF)

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GLI3 Antibody (600-401-694) in ICC/IF

Immunofluorescence Microscopy of Rabbit anti-Gli-3 antibody. Tissue: MCF-7 cell. Antigen retrieval: not required. Primary antibody: Gli-3 antibody and Anti-alpha-Tubulin at 5 µg/mL for 1 h at RT. Secondary antibody: Fluorescein secondary antibody at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear. Staining: Image (1) shows alpha-Tubulin staining as green fluorescent signal. Image (2) shows Gli-3 staining as red fluorescent signal and Images (3) shows both antibodies fluorescing using STED microscopy.

GLI3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

600-401-694

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500- 1:2,000

免疫组化（石蜡） (IHC (P))

0.5-5 µg/mL

免疫细胞化学 (ICC/IF)

Assay-dependent

酶联免疫吸附实验 (ELISA)

1:6,000-1:30,000

免疫沉淀 (IP)

1:500-1:2,000

产品规格

种属反应

Avian, Dog, Chicken, Chimpanzee, Human, Non-human primate, Xenopus

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity-purified antibody is directed against human Gli-3 protein. A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.

靶标信息

This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: GLI-Kruppel family member 3; GLI-Kruppel family member GLI3; GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome); GLI3 form of 190 kDa; GLI3 full length protein; GLI3 full-length protein; GLI3-190; GLI3FL; glioma-associated oncogene family zinc finger 3; neural specific DNA binding protein; Neural-specific DNA-binding protein xGLI3; oncogene GLI3; Transcriptional activator GLI3; xGLI-3; Zinc finger protein GLI3

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基因别名: ACLS; GCPS; GLI3; GLI3-190; GLI3FL; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV; XELAEV_18031191mg; xgli3