Invitrogen

GM130 Polyclonal Antibody, Alexa Fluor™ 647

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GM130 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GM-130 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a GM-130 Polyclonal Antibody, AlexaFluor 647 conjugate (Product # PA1-077-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

产品信息

PA1-077-A647

应用	建议稀释比	已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫细胞化学 (ICC/IF)	1-10 µg/mL	-

产品规格
种属反应	Human
已发表种属	Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide corresponding to residues G(368) Q V M E S V R Q L Q M E R D K(383) of human GM130
偶联物	Alexa Fluor™ 647
激发/发射光谱	查看光谱
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 1mg/mL BSA
内含物	0.05% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2663179

产品详细信息

PA1077A647 detects GM130 from human samples.

The PA1077A647 immunogen is a synthetic peptide corresponding to residues G(368) Q V M E S V R Q L Q M E R D K(383) of human GM130.

靶标信息

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A MST1-FOXO1 cascade establishes endothelial tip cell polarity and facilitates sprouting angiogenesis.

Nature communications

Kim YH,Choi J,Yang MJ,Hong SP,Lee CK,Kubota Y,Lim DS,Koh GY

PA1-077-A647 was used in Immunohistochemistry to delineate a crucial coupling between extracellular hypoxia and an intracellular reactive oxygen species-MST1-FOXO1 cascade in establishing endothelial tip cell polarity during sprouting angiogenesis.

Tue Feb 19 00:00:00 EST 2019

生物信息学

蛋白别名: 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; golgi autoantigen, golgin subfamily a, 2; Golgi matrix protein GM130; Golgin subfamily A member 2; Golgin-95; RP11-395P17.5; SY11 protein

基因别名: GM130; GOLGA2

UniProt ID: (Human) Q08379

Entrez Gene ID: (Human) 2801

分子生物学功能: membrane traffic protein

功能

protein glycosylation ER to Golgi vesicle-mediated transport microtubule nucleation asymmetric cell division negative regulation of autophagy negative regulation of protein binding COPII vesicle coating spindle assembly protein homotetramerization centrosome organization positive regulation of protein glycosylation Golgi ribbon formation Golgi disassembly spindle assembly involved in meiosis mitotic spindle assembly cell-cell adhesion

参与通路

protein binding microtubule binding protein kinase binding syntaxin binding importin-alpha family protein binding cadherin binding involved in cell-cell adhesion

GM130 Polyclonal Antibody, Alexa Fluor™ 647

产品信息

PA1-077-A647

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

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