Invitrogen

GOLT1B Polyclonal Antibody

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1 篇参考文献

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问题与答案

问题与答案

问题与答案

抗体检测数据 (2)
已发表图片 (1)

GOLT1B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GOLT1B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GOLT1B Antibody (PA5-103499) in IHC (P)

Immunohistochemistry analysis of GOLT1B in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GOLT1B polyclonal antibody (Product # PA5-103499) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

GOLT1B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GOLT1B Antibody in Western Blot (WB)

GOLT1B Antibody in Western Blot (WB)

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GOLT1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GOLT1B in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GOLT1B polyclonal antibody (Product # PA5-103499) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

GOLT1B Antibody in Western Blot (WB) — Western blot analysis of GOLT1B in various samples (Lane 1: mouse brain treated with blocking peptide, Lane 2: mouse brain, Lane 3: rat brain). Samples were incubated with GOLT1B polyclonal antibody (Product # PA5-103499).

GOLT1B Antibody in Western Blot (WB) — Figure 1 The mRNA and protein expression of GOLT1B was up-regulated in human breast cancer. The expression of GOLT1B in breast cancer tissues versus normal tissues was investigated using the datasets from (A) the GEPIA database, (B) the UALCAN database, and (C) the TCGA database. (D) The GOLT1B expression in 112 paired breast cancer tissues and adjacent normal tissues using the datasets from TCGA database. (E) The protein expression of GOLT1B in breast cancer tissues and normal breast tissues was evaluated through immunohistochemical tests from The Human Protein Atlas database. (F) Western blotting detecting GOLT1B expression in normal mammary epithelial cell line and breast cancer cell lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Immunogen image

GOLT1B Polyclonal Antibody

Immunogen Image GOLT1B Polyclonal Antibody

产品信息

PA5-103499

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

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免疫组化 (IHC)

1:50-1:200

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human GOLT1B(Accession Q9Y3E0), corresponding to amino acid residues G107-V138.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total GOLT1B.

靶标信息

GOT1B may be involved in fusion of ER-derived transport vesicles with the Golgi complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Germ cell tumor 2; Golgi transport 1 homolog B; hGOT1a; Putative NF-kappa-B-activating protein 470; Vesicle transport protein GOT1B

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基因别名: 2310061A22Rik; AA407874; AU024291; CGI-141; GCT2; GOLT1B; GOT1; GOT1A; GOT1B; HDCMA39P; RGD1310691; UNQ432/PRO793; YMR292W

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UniProt ID: (Human) Q9Y3E0, (Mouse) Q9CR60

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Entrez Gene ID: (Human) 51026, (Mouse) 66964, (Rat) 362460

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signal transducer activity lipase activity lipase

signal transduction protein transport vesicle-mediated transport positive regulation of I-kappaB kinase/NF-kappaB signaling lipid metabolic process transport