Invitrogen

GPR18 Polyclonal Antibody

Product Image Gallery

GPR18 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of GPR18 in formalin-fixed paraffin-embedded tissue section of human tonsil. Samples were incubated in GPR18 polyclonal antibody (Product # PA5-23218) using a dilution of 1:200 followed by HRP labeled anti-rabbit secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin.

GPR18 Antibody in Western Blot (WB) — Western blot analysis of GPR18 in human spleen lysate (4 µg/mL) in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen (6 µg/mL) and 4) rat spleen lysate (3 µg/mL). Samples were incubated in GPR18 polyclonal antibody (Product # PA5-23218).

GPR18 Antibody in Flow Cytometry (Flow) — Flow cytometry of GPR18 in THP-1 cells (blue) and a matched isotype control (orange). Samples were incubated in GPR18 polyclonal antibody (Product # PA5-23218) using a dilution of 1.0 µg/mL for 30 minutes at room temperature followed by a Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight™ 550 (Product # SA5-10033). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.

产品信息

PA5-23218

应用	建议稀释比	已发表文章
免疫印迹 (WB)	2-6 µg/mL	查看2篇已发表文章 2篇已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	1:200	-
免疫细胞化学 (ICC/IF)	2 - 10 µg/mL	-
流式细胞分析 (Flow)	Assay-Dependent	-

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide (2 ITLNNQDQPVPFNSSHPDEYK 22) of GPR18 protein.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2540743

靶标信息

The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. GPR18 is an orphan G protein-coupled receptor found at high levels in the testis, small intestines and cells associated with the immune system including lymphocytes, thymus and spleen. GPR18 has been found to be a receptor for endogenous lipid neurotransmitters, several of which also bind to cannabinoid receptors. Some ligands described to be GPR18 agonists or partial agonists include N-Arachidonoylglycine, Abnormal cannabidiol, O-1602, AM-251, Cannabidiol, delta 9-Tetrahydrocannabinol, N-arachidonoyl ethanolamine and Arachidonylcyclopropylamide. Antagonists include the synthetic cannabidiol related compounds CID-85469571 and O-1918.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ETS2 and microRNA-155 regulate the pathogenesis of heart failure through targeting and regulating GPR18 expression.

Experimental and therapeutic medicine

Li J,Su H,Zhu Y,Cao Y,Ma X

PA5-23218 was used in Western Blotting to investigate the underlying mechanism in heart failure (HF) using bioinformatics and experimental validation.