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GPXh/Glutathione peroxidase (algal) Polyclonal Antibody
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GPXh/Glutathione peroxidase (algal) Antibody (AS15-2882) in WB
10 µg of a total protein from Chlamydomonas reinhardtii extracted with 2% SDS/50 m M TRIS p H 6.8 + protease inhibitor cocktail were separated on 12% SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5% low-fat milk powder TBS + 0.1% Tween for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5,000 for 1 h at RT wit... View More
产品信息
AS15-2882
产品规格
种属反应
Algae
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from glutathione peroxidase protein sequence from Chlamydomonas reinhardtii.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Chlamydomonas reinhardtii
靶标信息
GPXH encodes a glutathione peroxidase-homologous, thioredoxin-dependent peroxidase that functions in the cellular defense against photooxidative stress in the green alga Chlamydomonas reinhardtii, with expression strongly and rapidly induced by singlet oxygen generated during high-light and photosensitizer exposure. The GPXH protein is predicted to be dual-targeted, localizing to both the chloroplast and the cytoplasm, consistent with a role in detoxifying reactive oxygen species and protecting membranes in multiple compartments. Functionally, GPXH catalyzes the reduction of hydrogen peroxide and organic (including lipid) hydroperoxides, using thioredoxin as the electron donor (and showing activity with plastidic TRXy as well as cytosolic TRXh1), thereby limiting hydroperoxide accumulation that can otherwise drive oxidative damage during photoinhibition.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AAEL012069; GPXH1
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