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Invitrogen
GSK3B Polyclonal Antibody
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GSK3B Antibody (PA5-77835) in WB
Western blot analysis of GSK3B in human cell line lysates with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with GSK3B polyclonal antibody (Product # PA5-77835) using a dilution of 1:1000 (2 hours at 4°C), followed by Donkey Anti-Rabbit HRP (1 hour at RT).
产品信息
PA5-77835
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta
偶联物
形式
Liquid
浓度
1.3 mg/mL
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
1 µg/mL of PA5-77835 was sufficient for detection of GSK-beta in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 47kDa.
靶标信息
GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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